NHAC/BMP复合物修复兔桡骨大段缺损及加强局部VEGF表达的研究  

Empirical study on the compound of NHAC and BMP on repairing bone defect and enhancing the expression of vascular endothelial growth factor(VEGF) in rabbts’ radial

作　　者：黄涛[1] 常祺[1] 黄昌林[1] 薛刚[1] 任洪峰[1] 赵琳[1] 鞠晓伟[1] 

机构地区：[1]中国人民解放军第150中心医院,河南洛阳471000

出　　处：《中医正骨》2009年第8期1-4,共4页The Journal of Traditional Chinese Orthopedics and Traumatology

摘　　要：目的:观察纳米级羟基磷灰石(NHAC)材料及其复合BMP后的骨修复能力和诱导生成血管能力。方法:制作兔桡骨大段骨缺损模型,分别以NHAC/BMP、NHAC修复,并设立空白对照组。术后6个月处死动物,进行大体观察、X线摄片、组织形态学检查(HE染色)、组织切片碱性磷酸酶染色(改良钙钴法染色)、成骨量分析(图像分析)、VEGF阳性细胞率检测(免疫组化染色)、VEGF阳性血管数检测(免疫组化染色)。结果:NHAC/BMP组、NHAC组及空白对照组间各项观测指标均差异显著,NHAC/BMP组优于NHAC组,NHAC组优于空白对照组(P<0.01)。结论:NHAC是一种很好的组织工程植骨材料,具有良好的骨传导性、生物相容性及降解性能。将其与BMP复合后,复合材料不仅骨修复能力进一步增强,其诱导血管生成能力也大大提高。Objective： To observe the ability of bone repairing and induced - blood vessel generation in the compound of Nano - hydroxyapatite- collagen （NHAC）and bone morphogenetic proteins （BMP）. Methode： The animal models of large segment radius bone defect were made by surgery in the New Zealand white rabbites, and were treated with the compound of NHAC and NHAC/BMP,by no implant as control. Six months later the animals were killed, changes were recorded by gross observations, X - ray examination, histopatbological examination （HE dyeing） , histological section alkaline phosphatase dyeing, bone formation analysis, detection on ratio of vascular endothelial growth factor （VEGF） -positive cells （Immunoreactivity）, count of VEGF -positive microvessels （Immunoreactivity）. Result： There had significant difference between three groups in all the detected items, the effects indicated that the ability of repairing bone defects and enhancing angiogenesis in group NHAC/BMP was better than other two groups ,and group NHAC far better than control group. Conclusions： NHAC was a wonderful osteoconductive biocompatible degradable artificial bone material ,when it was used to combine with BMP, the compound＇ s ability of repairing bone defects and enhancing angiogenesis advances.

关 键 词：修复外科学 骨缺损 纳米级羟基磷灰石(NHAC) 血管内皮细胞生长因子(VEGF) 骨形态发生 蛋白(BMP) 血管生成 

分 类 号：R687[医药卫生—骨科学]