高效液相色谱法同时测定动物源食品中22种兽药残留技术研究  

Study on simultaneous determination for 22 veterinary drug residues in animal derived food by high performance liquid chromatography

作　　者：赵亚华[1] 李勇[1] 何学芳[1] 

机构地区：[1]安徽省马鞍山市疾病预防控制中心,安徽马鞍山243000

出　　处：《中国卫生检验杂志》2009年第8期1707-1709,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立动物源食品（畜、禽、水产品）中22种兽药（磺胺、磺胺醋酰、磺胺噻唑、磺胺嘧啶、磺胺吡啶、磺胺氯哒嗪、磺胺甲基嘧啶、磺胺二甲基嘧啶、喹乙醇、呋喃唑酮、氯霉素、磺胺甲恶唑、磺胺甲噻二唑、磺胺-5-甲氧嘧啶、敌菌净、甲氧苄啶、磺胺二甲异恶唑、磺胺甲氧哒嗪、磺胺间甲氧嘧啶钠、苯酰磺胺、磺胺二甲氧嘧啶、磺胺喹恶啉）残留同时测定的高效液相色谱法。方法：优化22种兽药残留同时测定的高效液相色谱分析条件：色谱柱D iscovery C18,梯度变速洗脱,紫外检测波长270 nm。样品用乙腈-氯仿（10＋1）提取、正己烷脱脂,乙腈-氯仿（1＋2）反提取兽药,用流动相溶解定容,上机分析测定。结果：22种兽药在优化的色谱分析条件下分离效果较好,其线性范围较宽,并在此浓度范围内显良好的线性关系（r=0.99-0.9999）,在畜、禽、水产品中3个浓度添加水平平均回收率为61.2%-103.0%,相对标准偏差为1.0%-9.5%,最低检出浓度为0.004-0.018 mg/kg。结论：方法准确,重现性、精密度好,杂质干扰少,且简便快速,成本低,适用范围广,可满足兽药多残留分析要求。Objective： To develop a method based on high performance liquid chromatography（HPLC） for simultaneous determining residues of 22 veterinary drugs including Sulfanilamide,Sulfacetamide,Sulfathiazole,Sulfadiazine,Sulfapyridine,Sulfachlorpyridazine,Sulfamerazine,Sulfamethazine,Olaquindox,Furazolidone,Chloramphenicol,Sulfisomezol,Sulfamethizole,Sulfamethoxydiazine,Diaveridine,Trimethopim,Sulfisoxazole,Sulfamethoxypyridazine,Sulfamonomethoxine Sodium,Sulfabenzamide,Sulfadimethoxine and Sulfaquinoxaline in animal derived food（livestock,poultry,aquatic products）.Methods：Optimization of HPLC conditions for simultaneous determining 22 veterinary drugs： column Discovery C18,gradient and speed elution,UV detection at 270 nm.Samples are extracted with acetonitrile-chloroform（10＋1）,defatted with n-hexane,anti-extracted veterinary drugs with acetonitrile-chloroform（1＋2）,dissolved with mobile phase,analysised by HPLC.Results：Under the optimized HPLC conditions,separation of 22 veterinary drugs are better,linear ranges are wide,and good linear relationship（r=0.99～0.9999） in their concentration scope.Three levels of concentration are added to livestock,poultry,and aquatic product samples,the average recovery are 61.2%～103.0%,coefficient of variation was 1.0%～9.5%,the minimum detectable concentration are 0.004～0.018 mg/kg.Conclusion：This method is of accuracy,high repeatability,precision and less interferences from impurities,simple and fast.The cost is low and apply scope is wide.This method has met the requirement of analysis of veterinary drug multi-residues.

关 键 词：高效液相色谱法 同时测定 动物源食品中22种兽药残留 

分 类 号：O657.72[理学—分析化学]