固相萃取-高效液相色谱串联离子阱质谱法测定水中微囊藻毒素  

Determination of microcystins(MC_S) in drinking water by HPLC/MS

作　　者：陈静[1] 周倩如[1] 郭小琳[1] 张学煃[1] 

机构地区：[1]重庆市疾病预防控制中心,重庆400042

出　　处：《中国卫生检验杂志》2009年第8期1720-1721,1724,共3页Chinese Journal of Health Laboratory Technology

基　　金：重庆市医学科研项目(2008-2-97)

摘　　要：目的：为饮水中的微囊藻毒素（MCS）监测提供灵敏而可靠的分析方法。方法：水样经固相萃取柱（ENVI-C18,5 g/20 ml）富集,甲醇洗脱和浓缩,用Discovery C18色谱柱（250 mm×3 mm ID;5μm）分离MCS,离子阱质谱检测。结果：纯净水中加入的3种MC的回收率在89.5%-94.5%;RSD为1.25%-2.12%,检出限分别为MC-RR 0.060μg/L、YR0.058μg/L、LR 0.048μg/L。结论：建立的方法快速、准确,能满足测定饮水中MCS的要求。Objictive：To provide a sensitive and reliable method for the determination of Microcystins（MCS）in drinking water.Methods：The analyte was preconcentrated with ENVI-18 solid phsse extration（SPE）colum.The eluate was concentrated by N2 gas.The target compounds were determined by HPLC/MS.Results：The tanget compounds showed good linearity up to 2.0 μg/ml and detection limit from 0.048 to 0.060 μg/L.The proposed method was applied to pure water with the recovery in the range of 89.5%～94.5% and RSD from 1.25% to 2.12% for MC-RR,YR,LR.Conclusion：The proposed method is sensitive and precise for the determination of MCS in drinking water.

关 键 词：固相萃取(SPE) 高效液相色谱/离子阱质谱(HPLC/ITMS) 测定 水 微囊藻毒素(MCS) 

分 类 号：R123.1[医药卫生—环境卫生学]