固相萃取-高效液相色谱法同时测定大豆中5种除草剂  

Simultaneous determination of 5 herbicide residues in soybean by high performance liquid chromatography with solid phase extraction

作　　者：崔勇[1] 刘思洁[1] 张冠英[1] 张博[1] 王蕴馨[1] 李青[1] 

机构地区：[1]吉林省卫生监测检验中心,长春130062

出　　处：《中国卫生检验杂志》2009年第8期1790-1791,1811,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：建立大豆中5种除草剂多残留同时检测的高效液相色谱分析方法。方法：样品经甲醇提取,C18固相萃取小柱净化后,采用高效液相色谱方法分离,检测波长为230 nm。结果：5种除草剂（普杀特、苯达松、氟磺胺草醚、喹禾灵、2,4-滴丁酯）在0.5-10.0 mg/L范围内线性良好,回收率为77.9%-101.8%,相对标准偏差为1.24%-6.50%,方法的定量限为0.05-0.08 mg/kg,能达到国家有关上述除草剂残留限量的要求。结论：本法操作简便,准确可靠,为质量监控提供了参考。Objective： A high performance liquid chromatography（HPLC） method for simultaneous determination of 5 herbicides residues in soybean was developed and evaluated.Methods：The analytes in soybean were extracted with methanol.The extract was cleaned up using an SPE-C18 cartridge,separated and determined by HPLC with UV detector at a wavelength of 230 nm.Results：The linearity of 5 herbicides was good in the range of 0.510.0 mg/L.The recovery of the method was 77.9%101.8% and relative standard deviation（RSD） 1.24%6.50%.The limits of quantitation（LOQ） of the 5 herbicide residues were 0.050.08 mg/kg.Conclusion：The method is accurate,reliable and easy to be operated.It can be used for the determination of 5 herbicide residues in soybean.

关 键 词：除草剂 大豆 高效液相色谱法 固相萃取 

分 类 号：R115[医药卫生—公共卫生与预防医学]