LC-MS/MS法测定人血浆中青蒿素和磷酸萘酚喹的浓度  

LC-MS/MS simultaneous determination of artemisinin and naphthoquine phosphate in human plasma

作　　者：方翼[1] 王静[1] 潘志恒[1] 李玉珍[1] 李鹏飞[1,2] 顾景凯[2] 

机构地区：[1]北京大学人民医院药剂科,北京100044 [2]吉林大学药物代谢研究中心,长春130023

出　　处：《药物分析杂志》2009年第8期1264-1269,共6页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立高效液相色谱-质谱联用法测定人血浆中青蒿素和磷酸萘酚喹的浓度。方法:选用Zorbax extend-C18色谱柱,以甲醇-10mmol·L-1乙酸铵为流动相,样品用液-液萃取法处理后进样,选用Q-TrapTM型三重四极杆串联质谱仪的多重反应监测(MRM)扫描方式进行检测。结果:青蒿素和磷酸萘酚喹的线性范围分别为4.00～1000ng·mL-1和0.500～500ng·mL-1,最低定量限分别为4.00ng·mL-1和0.500ng·mL-1,精密度在92.3%～106.5%之间,日间和日内相对标准差均低于10.6%,相对偏差-4.6%～8.7%,方法提取回收率均大于75%,稳定性较好。结论:本研究所建立的方法快速、灵敏、专属性强、重现性高,可用于复方磷酸萘酚喹片药动学研究。Objective： Todevelop a LC-MS/MS method for the determination of the Artemisinin and Naphthoquine Phosphate in human plasma. Method： Artemisinin, Naphthoquine Phosphate and the internal standard were extracted from plasma liquid-liquid extraction, and separated on a separated on a Zorbax extend-C18 column using methanol-10 mM· L-1 ammonium acetate （80：20, v/v） as mobile phase by gradient elution. Detection was carried out by multiple reaction monitoring on a Q-TrapTM LC-MS/MS system. Results： The linear range of Artemisinin and Naphthoquine Phosphate were 4.00-1000 ng ·mL-1 and 0.500-500 ng ·mL-1 respectively; the limits of quantitation was 4.00 ng ·mL-1 and 0.500 ng ·mL-1 respectively. Intra- and inter-day precisions were both less than 15% and the relative deviations were in the range -4.6%-8.7%. The recoverieswere more than 75% and stabilities were good. Conclusion： The method is a rapid, sensitive, selective and reliable method for the determination of Artemisinin and Naphthoquine Phosphate in human plasma. The assay is applied to a pharmacokinetic study of the Complex Naphthoquine Phosphate tablets in healthy volunteers.

关 键 词：高效液相色谱-质谱联用法 复方磷酸萘酚喹片 青蒿素 磷酸萘酚喹 

分 类 号：R917[医药卫生—药物分析学]