高效液相色谱法测定人血浆中的维生素E烟酸酯  

HPLC determination of tocopheryl nicotinate in human plasma

作　　者：刘燕[1] 杨海霞[1] 张海燕[1] 王国强[1] 于盛茂[1] 

机构地区：[1]山东省生物药物研究院,济南250101

出　　处：《药物分析杂志》2009年第8期1336-1338,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立用高效液相色谱法测定维生素E烟酸酯血药浓度的方法。方法:用乙腈沉淀蛋白,正己烷提取血浆中的维生素E烟酸酯,离心后取上清液,氮气吹干,甲醇定量溶解后,进行高效液相色谱测定。色谱柱为DiamonsilODS(4.6mm×150mm,5μm)柱,流动相为甲醇,流速为1.5mL·min-1,柱温35℃,检测波长为220nm。结果:该方法在0.109～2.725μg.mL-1浓度范围内线性关系良好(r=0.9991,n=6)。高、中、低3种浓度的日内、日间RSD均小于5%,血样5d的稳定性RSD也小于5%,方法回收率在90.6%～99.9%之间。结论:本方法简便、定量准确,适用于人血浆中维生素E烟酸酯的测定,以及维生素E烟酸酯人体药代动力学、生物利用度和生物等效性的研究。Objective：To establish an HPLC method for the determination of tocopheryl nicotinate in human plasma.Method：Plasma protein was precipitated by the addition of acetonitrile.n-Hexane was used to extract tocopheryl nicotinate in the plasma.The resulting supernatant liquid after centrifuge was dried by nitrogen and was analyzed by the HPLC method after it was diluted quantitatively by methonel.The following chromatography conditions were used：The column was ODS-C18,mobile phase was methonel.The flow rate was 1.5 mL？min-1 and the column temperature was 35 ℃. The detective wavelength was 220 nm.Result： A good linearity was shown in the range of 0.109～2.725 μg？mL-1（r=0.9991,n=6）.The intra-day RSD and inter-day RSD were less than 5%.The stability of the five days was less than 5%.The absolute recovery was within 90.6%～99.9%.Conclusion：This method is simple,accuracy and applicable to study of pharmacokinetics,bioavailability and bioequiavailability of tocopheryl nicotinate.

关 键 词：维生素E烟酸酯 高效液相色谱法 血药浓度 

分 类 号：R917[医药卫生—药物分析学]