微波消化-固相萃取光度法测定中草药及制剂中的镉  

Spectrophotometric determination of dadmium in Chinese herbs and preparations by the solid phase extraction

作　　者：吴方评[1] 蒲艳春[1] 姚祖福[1] 杨宏健[1] 

机构地区：[1]湖南省怀化医学高等专科学校,怀化418000

出　　处：《药物分析杂志》2009年第8期1345-1347,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:合成了新试剂2-(2′-喹啉偶氮)-4,5-二甲基苯酚,并研究了其与镉的显色反应。方法:在pH=2.5的盐酸-邻苯二甲酸氢钾缓冲介质中,2-(2′-喹啉偶氮)-4,5-二甲基苯酚与镉反应生成2∶1稳定配合物,该配合物可被WatersPorapak(Sep-Pak-C18固相萃取小柱萃取,用乙醇(内含1%的乙酸)洗脱后用光度法测定。结果:在乙醇相中配合物λmax=550nm,ε=4.7×104L.mol-1.cm-1,镉含量在0～1.5μg·mL-1内符合比尔定律。结论:以上方法可用于中草药及制剂中镉含量的测定。Objective： Based onsynthesis of 2-（2′-quinolinylazo）-4,5-dimethylphenol,the color reaction of 2-（2′-quinolinylazo）-4,5-dimethylphenol with cadmium（Ⅱ） and the solid phase extraction of its colored complex with Waters Porapak？ Sep-Pak-C18 cartridge, a new method for the determination trace of cadmium（Ⅱ） was studied. Method： The presence of pH=2.5 HCl-potassium hydrogen phthalatebuffer solution. 2-（2′-quinolinylazo）-4,5-dimethylphenol can reacts with cadmium（Ⅱ） to form a stable 2：1 complex. The colored complex can be extracted by C18 cartridge and eluted by ethanol, then can be determined by spectrophotometry. Result： The molar absorptivity of the complex is 4.7×104 L.mol-1.cm-1 at 550 nm. Beer＇s law is obeyed in range of 0~1.5 mg.mL-1. Conclusion： This method can be applied to the determination of cadmium in Chinese herbs.

关 键 词：2-(2’-喹啉偶氮)-4 5-二甲基苯酚 镉 固相萃取光度法 

分 类 号：R917[医药卫生—药物分析学]