新加归肾丸对雷公藤致卵巢功能损害小鼠模型的影响  

Influence on Xinjia Guishen Bill to Reproductive Hypofunction Mice Reduced by GTW

作　　者：吴克明[1] 王家葵[1] 刘钺[1] 张黎鹏 谌婕[3] 童妍[4] 

机构地区：[1]成都中医药大学,四川成都610075 [2]西藏出入境检验检疫局,西藏拉萨850000 [3]四川雅安职业技术学院,四川雅安625000 [4]西南交通大学峨嵋分校,四川乐山614200

出　　处：《成都中医药大学学报》2009年第2期47-50,共4页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：四川省科技厅自然科学重点资助项目(04JY029-011-1);四川省教育厅科研基金资助项目(2004C004)

摘　　要：目的:以雷公藤多甙建立雌性育龄期小鼠生殖功能低下模型,用于探讨新加归肾丸改善生殖功能低下状态的作用机理。方法:将模型小鼠随机分为5组,每组15只,分别为新加归肾丸高、中、低剂量组、倍美力组、模型空白组,加上正常对照组共6组。连续给药3周,每日定时阴道上皮脱落细胞涂片,观察动情周期变化。第3周末取血、处死小鼠,采用放射免疫法检测血清E2、P、IGF-I水平,组织称重法计算卵巢、子宫指数,病理切片观察卵巢、子宫组织形态学变化。结果:与模型空白组相比,新加归肾丸组动情周期正常小鼠数量增加(P<0.05),卵巢、子宫的组织形态明显改善,高、中剂量组血清E2和P水平明显增高(P<0.01);模型组血清IGF-I与正常组相比显著升高(P<0.01),新加归肾丸各组IGF-I水平与正常组无显著性差异。结论:1、雷公藤多甙可造成雌性小鼠血清IGF-I异常升高,可能为雷公藤多甙致生殖功能损伤的机制之一;2、新加归肾丸能调节IGF-I在卵巢上的适量表达,促进卵泡发育和生长;3、新加归肾丸能改善子宫、卵巢的组织形态,调节卵巢激素水平,使紊乱的动情周期恢复正常。Objective： This study was to establish reproductive hypofunction model of female mature mice by GTW, and investigated the mechanism of improving reproductive hypofunction action of Xinjia Guishen Bill. Methods： The mice were randomLy divided into five groups, 15 in every group, including Xinjia Guishen Bill high, moderate, low - dose group, Premarin （conjugated estrogens tablets） group, model group , and normal control group, the total groups are 6. Successive administrating, smearing vaginal epithelial cell every day on fixture time, observing estrous cycle variation for 3 weeks, and then put all the mice to death. Serum E2, P and IGF- I level were measured by radioimmunoassay. Ovary and uterus index were measured by tissue -weight- assay. Histomorphology of ovary and uterus were observed by pathological slicing. Results： Compared with model group, the number of normal estrous cycle of Xinjia Guishen Bill group were increased （ P 〈 0. 01 ）, histomorphology of ovary and uterus were improved too. Serum E2, P level of high, moderate - dose groups were obviously increased （ P 〈 0. 01 ） . Compared with the normal, control group, serum IGF - I level of the model group increased anomaly, but Xinjia Guishen Bill group were normal increased lightly. Conclusion： Xinjia Guishen Bill could regulate some aspects of gonad axis, improve and enhance function of ovary, regulate hormone level in ovary. Thus improve low reproductive function.

关 键 词：新加归肾丸 雷公藤多甙 生殖功能 IGF-I 药理实验 

分 类 号：R271.41[医药卫生—中医妇科学] R711.51[医药卫生—中医学]