不同吸附剂对黄曲霉毒素B_1吸附效果的研究  

The Study of Binding Efficacy of Different Adsorbents on Aflatoxin B1

作　　者：李娟娟[1] 苏晓鸥[1] 

机构地区：[1]中国农业科学院农业质量标准与检测技术研究所,北京100081

出　　处：《中国畜牧兽医》2009年第8期5-10,共6页China Animal Husbandry & Veterinary Medicine

基　　金："十一五"国家科技支撑计划"饲料安全关键因子监测评价新技术研究"(2006BAD12B03)

摘　　要：作者通过体外试验和动物饲养试验,研究3种吸附剂对黄曲霉毒素B1(AFB1)的吸附效果。3种吸附剂分别是吸附剂A(主成分为酵母培养物提取物)、吸附剂B(主成分为水合铝硅酸盐)、吸附剂C(为复合物,主成分为酵母培养物及水合铝硅酸盐)。体外试验研究pH为2.0、6.0、8.0的磷酸盐缓冲液(PBS溶液)、人工胃液和肠液环境下3种吸附剂对AFB1的吸附能力,比较pH 8.0条件下3种吸附剂对AFB1的结合速率。结果表明,各种条件下吸附剂B的吸附能力最强。pH 8.0条件下,吸附剂B在10 min内对AFB1吸附率达到97.69%,且60 min内吸附率一直处在96.03%以上。吸附剂A和吸附剂C对AFB1的作用效果较吸附剂B弱。动物饲养试验选择240只1日龄健康雄性AA肉仔鸡,随机分为8个处理,每个处理3个重复,每个重复10只鸡,饲养周期21 d。结果表明,与基础组相比较,AFB1组(污染水平为98.8μg/kg)肉仔鸡生长性能和肝脏功能发生显著变化,吸附剂A、B、C均能不同程度地缓解AFB1对肉仔鸡生长性能和肝脏损伤方面的毒性作用,与吸附剂A、C相比,吸附剂B能更有效的防止肉仔鸡黄曲霉毒素B1中毒。结果提示,使用吸附剂B能很好的降低AFB1对肉仔鸡的危害程度。The aim of this paper was to study the efficacy of three absorbents to sorb aflatoxin B1 （AFB1）. The main component of product A is yeast product, the main component of product B is HSCAS, and product C is the compound of yeast product and HSCAS. In vitro trial, AFB1 binding capacity by three sorbents at different in vitro condition and AFB1 binding speed by three sorbents at pH 8.0 in vitro condition were compared. Product B showed the highest in vitro affinity for AFB1 , and the rate of sorption reached to 97.69% in 10 min and it remained over 96.03% in 60 min at pH 8.0. Product A and product C did not showed so efficacy as product B. In vivo trial, a total of 240 broilers were assigned to 8 treatments （3 pens of 10 broilers per treatment）. After 21 d, the aflatoxin-contaminated feed significantly decreased the growth performance and the liver function of broilers. The three products tested alleviated the adverse effects caused by aflatoxin, but compared with product A and C, product B could alleviate some of the toxic effects of AFB1 more effectively.

关 键 词：吸附剂 AFB1 吸附效果 肉仔鸡 

分 类 号：S852.44[农业科学—基础兽医学]