化学发光免疫分析法检测猪尿中莱克多巴胺残留  

Determination of Ractopamine(RAC) Residues in the Swine Urine by Chemiluminescence Immunoassay

作　　者：高彬文[1,2] 张素霞[1] 梁永红[2] 邢保松[2] 

机构地区：[1]中国农业大学动物医学院,北京100193 [2]河南农业科学院畜牧兽医研究所,郑州450002

出　　处：《中国畜牧兽医》2009年第8期27-30,共4页China Animal Husbandry & Veterinary Medicine

摘　　要：本试验运用化学发光免疫分析法（chemiluminescence immunoassay,CLIA）检测猪尿中莱克多巴胺（Ractopamine,RAC）的残留。检测限为0.01 ng/mL,检测范围为0.036~32.2 ng/mL。添加浓度分别为0.1、1、10 ng/mL,平均回收率为73.2%,批内平均变异系数为9.7%,批间平均变异系数为11.3%。而本研究所建立的化学发光免疫检测方法（CLISA）可以达到检测RAC残留的要求。A method was established for the determination of Ractopamine （RAC） residues in swine urine by indirect competitive chemiluminescence immunoassay （CLIA）. The limit of detection was 0. 01 ng/mL, and the detection ranged from 0. 036 to 32.2 ng/mL. At the fortified levels of 0.1, 1 and 10 ng/mL, mean recoveries was 73.2%, the intra-assay variation was 9.7%, and the inter-assay variation was 11.3 %.

关 键 词：化学发光 免疫分析 莱克多巴胺 猪尿 

分 类 号：S859.84[农业科学—临床兽医学]