培养基质中添加酯化葡甘露聚糖和DON对小鼠脾细胞生长和转化的影响  

Effect of the Adsorption of EGM to DON on Lymphocyte Conversion Ratio in Medium

作　　者：朱连勤[1] 李华[1] 朱风华[1] 隋菲菲[1] 常顺华[1] 

机构地区：[1]青岛农业大学动物科技学院,青岛266109

出　　处：《中国畜牧兽医》2009年第8期30-32,共3页China Animal Husbandry & Veterinary Medicine

基　　金：青岛市科技计划项目(08-3-3-31-nsh)

摘　　要：将体外培养的脾细胞分为5组,以RPMI-1640为基础培养基,第1组为空白对照组;第2组为阳性对照组,添加500μg/L DON;第3组、第4组和第5组在添加500μg/L DON的基础上分别添加0.05%、0.10%和0.15%的EGM。在培养72h后,测定各组培养基质中MDA含量、SOD活性、脾细胞存活率和淋巴细胞转化率。结果表明,第1组、第4组和第5组的脾细胞存活率、SOD活性极显著高于第2组(P<0.01);第1组、第4组和第5组的淋巴细胞转化率显著高于第2组(P>0.05);第2组培养液中MDA含量显著高于第1组、第4组和第5组(P<0.05);第1组、第4组和第5组各项指标差异不显著(P>0.05)。由此表明,EGM能很好地吸附DON,减轻DON对小鼠脾细胞的毒性作用;EGM在饲料中的适宜添加剂量为0.10%。The spleen cells were cultured in vitro were divided into five groups for seventy-two hours to study the adsorption of EGM to DON, RPMI-1640 was chosen as basal culture medium, nothing was added in the first group; 500 μg/L DON was added in the second group; 0.05% ,0.10% and 0. 15% EGM was respectively added in the third, the forth and the fifth group. The results showed that the survival rate of spleen cells, the activity of SOD of the first, the forth and the fifth group were significantly higher than the second group （P〈0.01）, the transformation rate of lymphocyte of the first, the forth and the fifth group were higher than the second group （P〈0.05）, the content of MDA of the second group was higher than the first, the forth and the fifth group （P〈0.05）, and the difference of all indices among the first, the forth and the fifth group were not significant（P〉0.05）. It concludes that EGM can effectively adsorb DON and relieve the toxic effect of DON to cul tured spleen cells in vitro, and the best additive dose of EGM in diet is 0.10%.

关 键 词：酯化葡甘露聚糖(EGM) 小鼠 呕吐毒素(DON) 淋巴细胞转化率 

分 类 号：S852.662[农业科学—基础兽医学]