胃康舒宁对大鼠慢性萎缩性胃炎癌前病变癌相关细胞因子的影响  

Impact of Weikang Shuning Decoction on cytokines involved in the development of gastric precancerous lesions in rats with chronic atrophic gastritis

作　　者：杨丽萍[1] 张文娴[1] 蒋时红[1] 刘旺根[2] 王琦[1] 王雪萍[1] 

机构地区：[1]河南中医学院基础医学院,河南省郑州市450003 [2]郑州大学基础医学院,河南省郑州市450001

出　　处：《世界华人消化杂志》2009年第19期1978-1981,共4页World Chinese Journal of Digestology

基　　金：河南省教育厅自然科学计划基金资助项目;No.2008A360021~~

摘　　要：目的:探讨胃康舒宁对胃癌前病变(PLGC)大鼠血清多种癌相关细胞因子的影响.方法:将60只Wistar大鼠随机分为4组:正常组、模型组、维酶素组和胃康舒宁组.采用饮用N-甲基-N-亚硝基-N-硝基胍(MNNG)建立大鼠胃癌前病变模型,用维酶素和胃康舒宁治疗12wk后,用放射免疫法检测各组大鼠血清中表皮生长因子(EGF)、转化生长因子-α(TGF-α)、生长激素(GH)、肿瘤坏死因子-α(TNF-α)、胰岛素样生长因子-Ⅱ(IGF-Ⅱ)、碱性成纤维细胞生长因子(bFGF)的水平.结果:与模型组大鼠相比,胃康舒宁组大鼠血清GH,TNF-α,IGF-Ⅱ水平显著增高(0.24±0.10μg/L vs 0.11±0.04μg/L,91.46±7.63μg/L vs 83.64±3.75μg/L,0.38±0.12μg/L vs 0.27±0.17μg/L,P<0.05或0.01),EGF,TGF-α,bFGF水平明显降低(0.40±0.11μg/L vs 0.56±0.15μg/L,0.021±0.006μg/L vs 0.029±0.012μg/L,1.44±0.37μg/L vs 3.26±0.45μg/L,P<0.05或0.01).结论:胃康舒宁对大鼠胃癌前病变具有良好的治疗和逆转作用,其疗效优于维酶素,而调节EGF,TGF-α,GH,TNF-α,IGF-Ⅱ,bFGF的分泌水平是其防治慢性萎缩性胃炎的作用机制之一.AIM： To investigate the impact of Weikang Shuning Decoction on cytokines involved in the development of gastric precancerous lesions in rats with chronic atrophic gastritis.METHODS： Sixty Wistar rats were randomly divided into four groups, namely, normaI control group, model control group, Vitacoenzyme group and Weikang Shuning decoction group. A rat model of gastric precancerous lesions was created by administration of N-methyl-N-nitroso-N-nitrosoguanidine （MNNG） via drinking water. After the rats were treated with Vitacoenzyme and Weikang Shuning Decoction for 12 weeks, respectively, the levels of serum EGF, TGF-α, GH, TNF-α, IGF-II and bFGF were determined by radioimmunoassay （RIA）. RESULTS： The levels of serum GH, TNF-α and IGF-Ⅱin the Weikang Shuning Decoction group were significantly higher that those in the model control group （0.24 ± 0.10 μg/L vs 0.11 ± 0.04 Hg/L, 91.46 ± 7.63 μg/L vs 83.64 ± 3.75 μg/L and 0.38 ± 0.12 μg/L vs 0.27 ± 0.17 μg/L, respectively, P 〈 0.05 or 0.01）. In contrast, the levels of serum EGF, TGF-α and bFGF in the Weikang Shuning Decoction group were significantly lower than those in the model control group （0.40 ± 0.11 μg/L vs 0.56 ± 0.15 μg/L, 0.021 ± 0.006 μg/L vs 0.029 ± 0.012 μg/L and 1.44 ± 0.37μg/L vs 3.26 ± 0.45 μg/L, respectively; P 〈 0.05 or 0.01）. CONCLUSION： Weikang Shuning Decoction has a good therapeutic effect against gastric precancerous lesions in rats, and its efficacy is superior to that of Vitacoenzyme. Regulation of serum EGF, TGF-α, GH, TNF-α, IGF-Ⅱand bFGF levels may be one of the mechanisms underlying the therapeutic effect of Weikang Shuning Decoction against gastric precancerous lesions.

关 键 词：胃康舒宁 慢性萎缩性胃炎 胃癌前病变 癌相关细胞因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]