诊断级超声辐照对人早孕绒毛细胞TNF-α及其p55型受体表达的影响  

Effect of diagnostic ultrasound irradiation on trophoblast TNF-α and tumor necrosis factor receptor p55 expression in first-trimester pregnancy

作　　者：福林[1] 额尔敦[2] 白石山[3] 王默琦[4] 

机构地区：[1]中国人民解放军第二五三医院妇产科,呼和浩特市010051 [2]中国人民解放军第二五三医院免疫中心,呼和浩特市010051 [3]中国人民解放军第二五三医院传染科,呼和浩特市010051 [4]中国人民解放军第二五三医院病理科科,呼和浩特市010051

出　　处：《临床超声医学杂志》2009年第8期534-536,共3页Journal of Clinical Ultrasound in Medicine

摘　　要：目的探讨诊断级超声剂量与人早孕绒毛肿瘤坏死因子仪（TNF—α）及其p55型受体（p55 TNFR）表达的关系，探寻诊断级超声诱导滋养层细胞凋亡的机制。方法拟进行人工流产的孕45～60d妇女60例，随机分为4组：即经腹对孕囊持续辐照10min、20min、30min组和空白辐照对照组。超声辐照后24h内行人工流产后取材，用免疫组化技术检测上述各组绒毛滋养层细胞TNF—α和p55 TNFR表达情况。结果超声辐照10min组绒毛滋养层细胞TNF-α和p55TNFR表达率与对照组比较差异无统计学意义；20min组和30min组TNF—α、p55 TNFR明显增加，分别为（61．94±6．34）％、（67．38±8．77）％和（65．39±5．96）％、（74．634±7．38）％，显著大于对照组（54．26±6．48）％、（61．56±9．23）％和10min组（55．54±2．82）％、（62．75±11．84）％，P〈0．01。结论诊断级超声持续辐照早孕孕囊组织大于10min，可引起绒毛滋养层细胞TNF—α和p55TNFR表达率增加，从而诱导滋养层细胞凋亡。Objective To investigate the relationship between ultrasonic dose TNF - α and p55 TNFR expression, and to explore the mechanism of trophoblasts cell apoptosis induced by diagnostic ultrasound. Methods Sixty first - trimester pregnant women were randomly devided into four groups. Then they were exposed with ultrasonic irradiation for 0 rain, 10 rain, 20 min, or 30 min. The chorionic villi were obtained within 24 hours after ultrasound irradiation. The expression of TNF - α and p55 TNFR in each group was detected by immunohistochemistry. Results Compared with control group, there was no significant difference of TNF - α and p55 TNFR expression in 10 min group. While the expression rate of TNF - α and p55 TNFR was significantly increased in20 rain group（61.94 ±26.34）% ,（67.38 ±28.77）% and 30 min group （65.39± 25.96）% ,（74.63 ±2 7.38 ） % （ P 〈 0.01 ）, which was more than control group （54.26 ±2 6.48 ） % , （61.56 ±2 9.23 ） % and 10 min group （55.54± 2 2.82） % , （62.75 ± 11.84）%. Conclusion Continuous irradiation over 10 minutes by diagnostic ultrasound may increase the expression rate of TNF - α and p55 TNFR in the first trimester pregnancy, then induce trophoblasts cell apoptosis.

关 键 词：超声检查 绒毛 肿瘤坏死因子Α 肿瘤坏死因子受体p55 

分 类 号：R445.1[医药卫生—影像医学与核医学] R285.5[医药卫生—诊断学]