固相萃取/LC-MS/MS测定尿液中吗啡类药物  

The determination of morphines in urine with LC-MS/MS by SPE

作　　者：闫志伟[1] 王玫[2] 潘冠民[2] 张大明[2] 

机构地区：[1]山西医科大学法医学院,山西太原030001 [2]北京市公安局法医鉴定中心,北京100192

出　　处：《中国法医学杂志》2009年第4期242-245,共4页Chinese Journal of Forensic Medicine

基　　金：国家科技支撑计划项目(2006BAK09B04)

摘　　要：目的 建立尿液中吗啡类药物的固相萃取／LC—MS／MS方法。方法采用OASIS MCX3cc（60mg）固相萃取柱进行提取，应用LC—MS／MS方法进行检测，运用保留时间和MRM方式对尿液中吗啡类药物及其代谢物进行定性定量分析。结果磷酸盐缓冲液pH4．0时，海洛因、6-MAM、可待因、吗啡、M3G的固相萃取回收率分别达64．33％-70．21％，96．95％～117．57％，83．60％～123．63％，68．82％～91．03％，94．64％～107．33％；最低检测限（LOD）分别为5、10、5、5、2pg，线性范围0．005～10μg／mL；相关系数分别为0．9998、0．9958、0．9992、0．9994、0．9997。结论本文所建方法，适用于尿液中吗啡类药物的分析。Objective To establish a solid phase extraction （SPE）/LC-MS/MS method for determination of morphines in human urine. Methods Morphines were extracted from human urine using OASIS MCX 3cc（60mg） extraction cartridges and detected by LC-MS/MS. Qualitative and quantitative analysis was obtained by retention time （Rt） and Multiple Reaction Monitoring （MRM） of LC-MS/MS. Results The recoveries for heroin, 6-MAM, codeine, morphine, M3G were 64.33% -70.21%, 96.95% - 117.57%, 83.60% - 123.63%, 68.82% -91.03%, 94.64% - 107.33% respectively, using phosphate buffer, pH4.0. Limits of Detection （LOD） were 5pg for M3G, 10pg for morphine, 5pg for codeine, 5pg for 6-MAM and 2pg for heroin. The linear range is 0. 005 - 10μg/mL. The correlation coefficient of heroin, 6-MAM, codeine, morphine, M3G were 0. 999 7,0. 999 4,0. 999 2,0. 995 8,0. 999 8 respectively. Conclusion The results of this study suggest that the method is simple, rapid and accurate for quantitative analysis of morphines and their metabolites in human urine.

关 键 词：法医毒物分析 吗啡类药物 固相萃取 LC—MS/MS 尿液 

分 类 号：D919[医药卫生—法医学]