兔出血症病毒在体外诱导细胞凋亡  

Studies on the Apoptosis of RK13 Cells Induced by Rabbit Hemorrhagic Disease Virus

作　　者：倪征[1] 魏威[1,2] 刘光清[1] 陈柳[1] 余斌[1] 云涛[1] 华炯钢[1] 李双茂[1] 

机构地区：[1]浙江省农业科学院病毒学与生物技术研究所,浙江杭州310021 [2]甘肃农业大学动物医学院,甘肃兰州730070

出　　处：《病毒学报》2009年第4期316-317,共2页Chinese Journal of Virology

基　　金：国家自然科学基金(30670074;30870114和30800045);浙江省自然科学基金(Y307124);家畜疫病病原生物学国家重点实验室开放基金资助

摘　　要：The apoptosis of RK13 cells induced by RHDV was investigated with DAPI staining,DNA ladder,Caspase 3 activity and flow cytometry,etc.The results showed that nuclear staining of infected cells with DAPI showed gradually morphological changes of the nuclei.As shown in the paper,a canonic oligonucleosome-sized DNA ladder was observed in cells harvested at 24h,48h and 72h post-infection,confirming that DNA fragmentation was induced by RHDV infection.The results of flow cytometry showed that about 63 % of cells were in apoptosis at 48h post-infection.Besides,we also demonstrated that the activation of Caspase 3 occurred during the infection process.In conclusion,our results showed that apoptosis in RHD might be determinant in the development of the pathogenesis of RHD.The apoptosis of RK13 cells induced by RHDV was investigated with DAPI staining, DNA ladder,Caspase 3 activity and flow cytometry, etc. The results showed that nuclear staining of infected cells with DAPI showed gradually morphological changes of the nuclei. As shown in the paper, a canonic oligonucleosome-sized DNA ladder was observed in cells harvested at 24h, 48h and 72h post-infection, confirming that DNA fragmentation was induced by RHDV infection. The results of flow cytometry showed that about 63 % of cells were in apoptosis at 48h post-infection. Besides, we also demonstrated that the activation of Caspase 3 occurred during the infection process. In conclusion, our results showed that apoptosis in RHD might be determinant in the development of the pathogenesis of RHD.

关 键 词：兔出血症病毒 RK13细胞 凋亡 CASPASE-3 

分 类 号：Q813[生物学—生物工程] S858.291[农业科学—临床兽医学]