落新妇苷对大鼠肾脏缺血再灌注损伤的保护作用  

Protective effects of astilbin on renal ischemia-reperfusion injury in rats

作　　者：宋少华[1] 沈筱芸[2] 刘芳[1] 唐乙[2] 王振猛[3] 傅志仁[1] 

机构地区：[1]第二军医大学长征医院器官移植科,上海200003 [2]第二军医大学免疫学研究所,上海200433 [3]第二军医大学东方肝胆外科医院麻醉科,上海200433

出　　处：《中西医结合学报》2009年第8期753-757,共5页Journal of Chinese Integrative Medicine

基　　金：国家自然科学基金资助项目(No.30772045);上海市卫生局青年科研基金资助项目(No.2008Y085)

摘　　要：目的:探讨落新妇苷对大鼠肾脏缺血再灌注(ischemia reperfusion,IR)损伤的保护作用。方法:将24只健康雄性SD大鼠随机分成假手术组、模型组和落新妇苷组,每组8只。模型组和落新妇苷组大鼠用于制作肾脏IR模型。自术前3d开始,落新妇苷组大鼠腹腔注射12mg/mL落新妇苷30mg/kg,1次/d;模型组大鼠腹腔注射等量生理盐水。于再灌注6h后检测各组大鼠血清尿素氮(blood urea nitrogen,BUN)和肌酐(serum creatinine,SCr)水平,并观察肾组织形态学变化;半定量逆转录聚合酶链式反应及蛋白印迹法检测肾组织内单核细胞趋化蛋白1(monocyte chemoattractant protein-1,MCP-1)mRNA及蛋白表达水平;酶联免疫吸附测定法检测各组大鼠血清白细胞介素6(interleukin-6,IL-6)和白细胞介素1β(interleukin-1β,IL-1β)含量。结果:再灌注6h后与模型组相比,落新妇苷组大鼠BUN和SCr水平均显著降低(P<0.01),大鼠肾小球及肾小管上皮细胞损伤明显减轻,肾组织内MCP-1mRNA(P<0.05)和蛋白表达下降,血清IL-6和IL-1β含量下降(P<0.01)。结论:落新妇苷能够有效减轻肾脏IR损伤,其保护作用可能与抑制IR后趋化因子MCP-1及细胞因子IL-6和IL-1β的产生有关。Objective： To investigate the protective effects of astilbin on renal ischemia-reperfusion （IR） injury in rats. Methods： Twenty-four male SD rats, two months old, were randomly allocated into three groups, shamoperated group （n=8）, untreated group （n=8） and astilbin group （n=8）. Rats in the untreated group and the astilbin group underwent temporary renal artery occlusion to induce IR injury. The rats in the astilbin group were intraperitoneally injected with 12 mg/mL astilbin at a dose of 30 mg/kg from 3 day before IR injury until to be sacrificed once per day, and rats in the untreated group were injected with equal volume of normal saline at the same time. After 6-hour reperfusion, blood urea nitrogen （BUN） and serum creatinine （SCr） and histological changes of the renal tissues were detected to evaluate renal injury. Expressions of monocyte chemoattractant protein-1 （MCP-1） mRNA and protein in the renal tissues and the serum contents of interleukin-6 （IL-6） and IL-1β were also measured with semi-quantitative reverse transcription-polymerase chain reaction, Western blotting or enzyme-linked immunosorbent assay. Results： Compared with the untreated group, BUN and SCr levels were significantly decreased in the astilbin group after 6-hour reperfusion （P〈0.01）, and similar results were also found in histological examination. The expressions of MCP-1 mRNA and protein in renal tissues in the astilbin group were lower than those Jn the untreated group. The serum contents of IL-6 and IL-1β were decreased in the astilbin group as compared with the untreated group （P〈0.01）. Conclusion. Astilbin can ameliorate kidney IR injury in rats by inhibiting the production of chemokine MCP-1 and cytokines IL-6 and IL-1β.

关 键 词：落新妇苷 再灌注损伤 肾脏 大鼠 

分 类 号：R285.5[医药卫生—中药学]