Reeler小鼠海马齿状回形态结构与组织化学研究  

Morphological and histochemical study of hippocampal dentate gyrus in reeler mice

作　　者：邓锦波[1] 王桂敏[2] 赵善廷[3] 范文娟[1] 李明善[1] 

机构地区：[1]河南大学医学院神经生物学研究所,河南大学神经生物学实验室,开封475004 [2]山东大学医学院生理教研室,济南250012 [3]兰州大学生命科学学院,兰州730000

出　　处：《解剖学报》2009年第4期522-526,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30670688和30771140);河南省科技厅国际合作项目资助(0646630014);河南省教育厅项目资助(2007180008)

摘　　要：目的分析reelin基因突变小鼠,即reeler小鼠海马锥体细胞和颗粒细胞的组织化学特征,了解Reelin缺乏对海马皮质发育的影响,为认识Reelin功能提供形态学证据。方法用免疫荧光双重标记法,分别标记野生型和reeler小鼠海马锥体细胞、颗粒细胞和苔藓细胞;结果Reeler小鼠海马皮质板发育明显障碍,表现为锥体层和颗粒层细胞扩散,海马片层状细胞构筑紊乱。颗粒层细胞明显增殖并向门区迁移,以致颗粒层与门区的界限消失,呈鼓槌状结构。结论Reelin作为神经细胞迁移的终止信号和细胞增殖的调控信号对神经细胞迁移、发育过程中皮质板的片层化,特别是对调节颗粒细胞的增殖有明显影响。Objective In order to investigate the influence of reelin deficiency on the hippocampal development, the histochemical characteristics of hippocampal pyramidal cells and granule cells of reeler mice were analyzed, therefore, reelin＇ s function would be better understood with more morphological evidences. Methods With immunofluorescent double labeling, the pyramidal cells, granule cells and mossy cells in hippocampi between wild type and reeier mice were labeled. Results The development and lamination of hippocampal cortical plate were in disorder. Pyramidal cells and granule cells dispersed, and moreover, granule cells proliferated rapidly and migrated into hilus, so that the bound between granule layer and hilus disappeared. Conclusion As a stop signal and regulatory signal, reelin plays important roles in neuronal migration of developing cortical plate, especially in the regulation of granule cell proliferation.

关 键 词：REELIN 皮质板 海马 组织化学 reeler小鼠 

分 类 号：R322[医药卫生—人体解剖和组织胚胎学]