槲皮素和乙酸龙脑酯对细菌脂多糖诱导流产小鼠子宫组织IFN-γ/IL-4和巨噬细胞的影响  

Influence of quercetin and bornyl acetate on ratio of IFN-γ/IL-4 and macrophages in uteri of LPS-induced abortion mice

作　　者：王晓丹[1,2] 马爱团[2] 史万玉[1] 姜国均[2] 钟秀会[1] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]河北农业大学中兽医学院,河北定州073000

出　　处：《解剖学报》2009年第4期637-641,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30571361)

摘　　要：目的探讨小鼠子宫组织中巨噬细胞在早期胚胎丢失中的意义,研究槲皮素和乙酸龙脑酯对细菌脂多糖（LPS）诱导流产孕小鼠的保胎效果,和对母胎界面免疫平衡的调节作用。方法选用LPS尾静脉注射（0.10μg/鼠）,制造小鼠流产模型,孕4~7d分别口服不同保胎药物,用药前后检测各组（n=10）小鼠子宫组织干扰素γ/白细胞介素4（IFN-γ/IL-4）的含量和巨噬细胞数量的变化。结果LPS促流产后,子宫匀浆中IFN-γ含量升高,IL-4含量降低,IFN-γ/IL-4的比值升高,与对照组比较差异极显著;孕小鼠子宫壁巨噬细胞数量显著增多。预先口服槲皮素和乙酸龙脑酯可不同程度地抑制LPS的作用,其中以槲皮素和乙酸龙脑酯联合使用组的保胎效果最为明显,IFN-γ/IL-4的比值基本达到正常水平,巨噬细胞数量降至16.199±0.802,较LPS流产模型组差异极显著。结论小鼠子宫组织中IFN-γ/IL-4的比值升高和巨噬细胞数量增多与早期胚胎丢失关系密切,槲皮素和乙酸龙脑酯能调节子宫局部的免疫微环境,从而起到一定的促孕保胎作用。Objective To investigate significance of interferon-T/interleukin-4（IFN-γ/IL-4） and macrophages in uterus in early embryo loss （or resorption）, and to elucidate the anti-abortive effect and the immunological modulation at maternal-fetal interface with quercetin and bornvl acetate. Methods Lipopolysaccharide （LPS）（0.10μg/mouse）was injected via tail vein in order to induce abortion in 7-day-gestation mice which received quercetin and borud acetate at days 4-7 of gestation. Levels of IFN-γ and IL-4 in uterus lysate superuatant were measured using enzyme-linked immuno-absorbent assay （ELISA）, and uterine macrophages of each group （ n = 10） were detected by immunohistochemistry. Results The amount of macrophages was much higher in the uteri of LPS-induced abortion mice than that in control mice. The abortion rate of mouse declined to certain level. The therapy of quercetin combined with boruyl acetate reversed LPS-induced abortion. Conclusion The increase of IFN-γ/IL-4 and the amount of maerophages in the LPS-treated mouse uterus is associated with the embryo loss, and quercetin and boruyl acetate have the anti-abortive effect through modulation of maternal-fetal interface immunity balance.

关 键 词：胚胎丢失 细菌脂多糖 巨噬细胞 干扰素γ 白细胞介素4 免疫组织化学 小鼠 

分 类 号：S853.7[农业科学—临床兽医学]