围着床期小鼠卵巢黄体生成素受体和血管内皮生长因子表达的免疫组织化学研究  

Immunohistochemical study for the expression of LHR and VEGF on the ovary of mice during peri-implantation

作　　者：石运芝[1] 魏丽华[1] 葛丽[1] 杜辉[1] 宋文刚[1] 

机构地区：[1]泰山医学院组织胚胎学教研室

出　　处：《解剖学报》2009年第4期647-650,共4页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30872322);山东省自然科学基金资助项目(Y2007C142);山东省教育厅科技发展计划资助项目(J07YD05)

摘　　要：目的探讨黄体生成素受体（LHR）和血管内皮生长因子（VEGF）在围着床期小鼠卵巢中的生物学作用。方法应用免疫组织化学SABC法和图像分析,检测LHR与VEGF在昆明小鼠（n=28）动情期、孕1d、孕4d和孕6d卵巢中的分布状况和变化规律。结果LHR与VEGF免疫阳性细胞具有相似的分布和变化规律。在动情期,较大的卵泡周围的基质细胞呈强阳性着色,与其他组相比,差异显著（P＆lt;0.05）。随着妊娠的继续,颗粒黄体细胞的着色逐渐增强,在孕6d时表达最强。在动情期和孕1d,部分血管内皮和血细胞分别呈VEGF和LHR强阳性着色。从孕1d开始,卵泡膜细胞呈LHR阳性着色。结论LHR和VEGF的表达与围着床期小鼠卵巢中卵泡的发育、排卵和黄体形成过程密切相关。Objective To explore the biological effects of the luteinizing hormone receptor （LHR） and vascular endothelial growth factor （VEGF） on the ovary of mice during peri-implantation. Methods The immunohistoehemistry SABC method and image analysis were used to study the distribution and changes of the LHR and VEGF in Kunming mouse（ n = 28） ovary during estrous, pregnancy of day 1, day 4 and day 6 stage. Results The expression of LHR-immunoreactive substance and that of VEGF-immunoreaetive substance had the same distribution and changes. Compared with other groups, the level of LHR- immunoreactive substance and that of VEGF-immunoreaetive substance increased highly on the stroma cells around larger growing follicles in estrous group （ P 〈 0.05）. Along with the pregnancy, the positive immunostaining for LHR and VEGF increased gradually on the granulosa lutein cells, and reached the highest level on day 6 of pregnancy. Positive immunostaining for LHR or VEGF on some endothelia and blood cells were observed in day 1 of pregnancy or estrous group respectively. Form day 1 of pregnancy, the theea cells had positive immunostaining for LHR. Conclusion The expression of LHR and VEGF is closely related with the process of follicle growing, ovulation and corpus luteum formation.

关 键 词：黄体生成素受体 血管内皮生长因子 围着床期 卵巢 免疫组织化学 小鼠 

分 类 号：R321.3[医药卫生—人体解剖和组织胚胎学]