溴苯腈对离体小鼠肝组织线粒体膜电位和呼吸控制率的影响及N-乙酰半胱氨酸的干预作用  

Effect of bromoxynil on membrane potent ial and respiratory control rate in isolated mitochondria from mice liver and intervention effect of NAC

作　　者：管霞飞[1] 赵光举[1] 蔡旗旗[1] 王志翊[1] 卢中秋[1] 邱俏檬[1] 洪广亮[1] 梁欢[1] 

机构地区：[1]温州医学院附属第一医院急诊科,325000

出　　处：《中华劳动卫生职业病杂志》2009年第8期472-475,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：浙江省医学扶植重点建设学科计划项目（07-F04）;浙江省医药卫生科学研究基金计划（20078144）

摘　　要：目的探讨溴苯腈对离体小鼠肝组织线粒体膜电位及呼吸控制率（RCR）的影响及N-乙酰半胱氨酸（NAC）的干预作用。方法采用差速离心法分离提取ICR雄性小鼠肝组织线粒体，随机分成正常对照组、溴苯腈染毒组、NAC保护组，用氧电极法测线粒体Ⅲ态呼吸速率（S3）、IV态呼吸速率（S4）、RCR；用阳离子荧光羰花青染色剂（JC-1）染色，共聚焦荧光显微镜观察线粒体膜电位变化。结果与正常对照组比较，溴苯腈染毒组的S3为（0．03±0．008）nanoatomsoxygen·mg^-1·min-1RCR为1．820±0．181，NAC保护组的RCR为4．253±0．210，均明显降低，溴苯腈染毒组的S4[（O．017±0．004）nanoatomsoxygen·mg^-1·min^-1明显升高，差异均有统计学意义（P〈0．01）；与溴苯腈染毒组比较，NAC保护组的s，为（0．046±0．005）nanoatomsoxygerdmg^-1·min^-1]、RCR（4．253±0．210）明显升高，S4[（0．011±0．001）nanoatomsoxygen·mg^-1·min^-1明显降低，差异均有统计学意义（P〈0．01）。共聚焦荧光显微镜观察，溴苯腈染毒组线粒体红色荧光明显减弱甚至消失，NAG保护组线粒体红色荧光较溴苯腈染毒组增强，但较正常对照组弱，3组间差别明显。结论离体小鼠肝组织线粒体经溴苯腈染毒后，线粒体RCR与膜电位明显下降；经NAC预处理后，可有效提高溴苯腈染毒线粒体的RCR与膜电位，对线粒体有较好的保护作用。Objective To demonstrate the effect of bromoxynil on membrane potential and respiratory control rate （RCR） in isolate mitochondria from mice liver tissue in viro and the intervention of NAC. Methods The mitochondrial was randomized to control group, bromoxynil-poisoned group and NAC-prospected group. S3,S4 and RCR of the mitochondria in each sample was detected by the method of oxygen electrede.Each sample was stained by JC-1 and the changes of membrane potential of mitochondria were observed under fluo- rescence microscope. Results The S3 [（0.031 ±0.008）nanoatoms oxygen, mg-1·min-1）],RCR （1.820±0.181） of bromoxynil-poisoned group and RCR （4.253±0.210） of NAC-prespected group were significantly lower than those of control group （P〈0.01） ;the S4[（0.017±0.004） nanoatoms oxygen-mg-1·min-1） of NAC-prospected group was significantly higher than control group （P〈0.01）.The S3[（0.046±0.005） nanoatoms oxygen, mg-1·min-1）] and RCR of NAC-prospected group were significantly higher than group B （P〈0.01）, S4 [（0.011±0.001） nanoatoms oxygen.mg-1·min-1）] of NAC-prospected group was significantly lower than bromoxynil-poisoned group （P〈 0.01）. Observaion under fluorescence microscope： the red fluorescence of mitochondria was dim or disappearred in bromoxynil-poisoned group while brightened in NAC-prospected group but still dimmer than control group Conclusion In vitro, the mitochondrial RCR and the mitochondrial membrane potential are decreased after the mitochondria is incubated with bromoxvnil, and NAC could improve it.

关 键 词：腈类 乙酰半胱氨酸 线粒体 肝 膜电位 

分 类 号：R686[医药卫生—骨科学]