家族性肾阳虚的基因功能富集分析  

Gene Function Enrichment Analysis of a Pedigree with Typical Kidney-Yang Deficiency Syndrome

作　　者：刘明[1] 丁维俊[2] 蔡欣[3] 张天娥[2] 李炜弘[2] 王米渠[2] 

机构地区：[1]四川大学华西医院生物治疗国家重点实验室,信号转导及分子靶向治疗研究室,成都610041 [2]成都中医药大学,成都610075 [3]成都铁路中心医院内分泌科,成都610081

出　　处：《中国生物化学与分子生物学报》2009年第8期747-752,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.90209013, No.30528029)~~

摘　　要：在临床调查中筛选出一个典型肾阳虚证家系,选取典型肾阳虚证患者3例及家系内正常人3例进行表达谱芯片试验.芯片分析结果显示,肾阳虚证者表达上调基因106条,下调基因16条.通过基因功能分类及深入的基因功能富集分析发现,肾阳虚证与GnRH信号通路及氧化磷酸化密切相关(P≤0.05),从而为肾阳虚证本质的研究提供了新的思路,但具体的机制有待深入研究.A typical deficiency-cold syndrome pedigree were analyzed based on clinical investigation, and microarrays were made to examine expression profile of kidney-yang deficiency syndrome. Compared with normal members in the family, members with kidney-yang deficiency syndrome showed that there were 106 genes up-regulated and 16 down-regulated by at least 1.5 fold. Gene classification and gene function enrichment analysis of differentially expressed genes indicated that kidney-yang deficiency syndrome was closely associated with GnRH signaling pathway and oxidative phosphorylation （ P ≤ 0.05）, but the exact mechanisms keeps unknown.

关 键 词：肾阳虚证 基因功能富集分析 家系 氧化磷酸化 GnRH信号通路 

分 类 号：Q78[生物学—分子生物学] S853.33[农业科学—临床兽医学]