Development and application of tumor-targeting magnetic nanoparticles FA-StNP@Fe_2O_3 for hyperthermia  

作　　者：ZHENG YuanQing TONG ChunYi WANG Bei XIE Ying LIAO HongDong LI Dan LIU XuanMing 

机构地区：[1]State Key Laboratory of Chemo/Biosening and Chemometrics,Hunan University, Changsha 410082, China [2]State Key Laboratory of Chemo,Research Center of Biological Energy Sources and Biomaterial,Hunan University, Changsha 410082, China [3]State Key Laboratory of Chemo/Institute of Life Science and Biotechnology,Hunan University, Changsha 410082, China

出　　处：《Chinese Science Bulletin》2009年第17期2998-3004,共7页

基　　金：Supported by the Key Program for Science and Technology of Hunan Province (Grant No. 03NKY1001) ;Key Construction Program of the National "985" Project

摘　　要：Modified magnetic starch nanoparticles (FA-StNP@Fe2O3) were synthesized by conjugating folic acid (FA-PEG-NH2) onto the surface of magnetic starch nanoparticles (StNP@Fe2O3) prepared by reverse microemulsion method. The synthesized FA-StNP@Fe2O3 was investigated by transmission electron microscopy and zeta potential analysis. The average size of its well dispersed particles was 250 nm. The iron concentration of 2 mg/g was detected by phenanthroline method. Placing FA-StNP@Fe2O3 nanoparticles in the alternating magnetic field for 30 min resulted in an increase in the suspension temperature from ambient temperature (37℃) to a value between 42℃ and 43℃. Co-cultured nanoparticles and Hela cell line or normal HUEC-12 cell line, and the biological effects at the cellular level were investigated in the alternating magnetic field using MTT assay, Hochest-PI double staining and flow cytometry analysis. Experimental results showed that FA-StNP@Fe2O3 within acertain concentration range has no obvious effect on cell proliferation. When treated in the magnetic field, apoptosis rate on Hela induced by FA-StNP@Fe2O3 was 13.4%. Prussian blue staining analysis confirmed that the nanoparticles modified with folic acid had improved ability in tumor cell-targeting, and therefore, potential applications in biomedical and magnetocaloric areas. It is expected be applied in tumor targeting therapy in the near future.Modified magnetic starch nanoparticles （FA-StNP@Fe2O3） were synthesized by conjugating folic acid （FA-PEG-NH2） onto the surface of magnetic starch nanoparticles （StNP@Fe2O3） prepared by reverse microemulsion method. The synthesized FA-StNP@Fe2O3 was investigated by transmission electron microscopy and zeta potential analysis. The average size of its well dispersed particles was 250 nm. The iron concentration of 2 mg/g was detected by phenanthroline method. Placing FA-StNP@Fe2O3 nanoparticles in the alternating magnetic field for 30 min resulted in an increase in the suspension temperature from ambient temperature （37℃） to a value between 42℃ and 43℃. Co-cultured nanoparticles and Hela cell line or normal HUEC-12 cell line, and the biological effects at the cellular level were investigated in the alternating magnetic field using MTT assay, Hochest-PI double staining and flow cytometry analysis. Experimental results showed that FA-StNP@Fe2O3 within acertain concentration range has no obvious effect on cell proliferation. When treated in the magnetic field, apoptosis rate on Hela induced by FA-StNP@Fe2O3 was 13.4%. Prussian blue staining analysis confirmed that the nanoparticles modified with folic acid had improved ability in tumor cell-targeting, and therefore, potential applications in biomedical and magnetocaloric areas. It is expected be applied in tumor targeting therapy in the near future.

关 键 词：应用 铁纳米粒子 Hela细胞 透射电子显微镜 流式细胞仪分析 热疗 反相微乳液法 交变磁场 

分 类 号：Q255[生物学—细胞生物学] TQ110[化学工程—无机化工]