枇杷叶中熊果酸诱导细胞凋亡的研究  

Study on the Apoptosis Induced by Ursolic Acid from Loquat Leaves

作　　者：邵敬伟[1] 王涛 林凤屏[1] 陈剑锋[3] 郭养浩[1] 

机构地区：[1]福州大学化学化工学院,福建福州350108 [2]郑州博赛生物技术股份有限公司,河南郑州450016 [3]福州大学生物科学和工程学院,福建福州350108

出　　处：《药物生物技术》2009年第4期355-359,共5页Pharmaceutical Biotechnology

基　　金：福建省科技厅青年人才创新项目(2007F0347);福建省教育厅项目(JA08004)

摘　　要：探讨枇杷叶中熊果酸(UA)对人肝癌HepG2、人胃癌MGC803、BGC823以及人乳腺癌M231细胞致凋亡作用及其对凋亡相关的半胱天冬酶-3活性(Caspase-3)的影响。方法:不同浓度(10、20、40、50、80μmol/L)的UA作用细胞,采用噻唑蓝(MTT)比色法检测细胞增殖,普通光镜和AO/EB荧光染色法观察细胞凋亡形态学变化,琼脂糖凝胶电泳检测细胞凋亡梯度,比色法检测细胞凋亡过程中Caspase-3活性变化。结果显示不同浓度的UA组细胞的生长与对照组相比均受到不同程度的抑制,细胞增殖抑制率具有剂量依赖关系;普通光镜观察显示,给药组部分细胞呈典型的凋亡表现;AO/EB荧光染色可见核浓缩及核碎裂等典型的细胞凋亡特征;琼脂糖凝胶电泳可见典型的梯状条带;不同浓度UA组细胞中Caspase-3酶活性较对照组明显升高(P<0.05),其作用具有剂量依赖性。结论:UA可诱导上述四种癌细胞的生长,诱导细胞凋亡发生,Caspase-3的激活在UA诱导细胞凋亡机制中起到重要作用。To investigate the anti-proliferation and apoptosis effects of Ursolic Acid （UA） from loquat leaves on hepatocarcinoma Cell HepG2, human gastric carcinoma cell MGCS03, BGC-803, and human breast carcinoma cell M231 cultured in vitro and the activity of caspase-3. Different concentrations of UA （10.20.40.50.80 μmol/L） were used to treat the cells. Thiazole blue colormetry was employed to detect the effects of UA on cell proliferation. After being treated by UA, the morphology of cells and nuclei was observed under light microscope and fluorescence microscope respectively. The agarose gel e lectrophoresis analysis revealed DNA cleavages. The enzyme activities of Caspas-3 were determined by colorimetry method. Results. Compared with the control group, the cell growth in the experimental group was depressed significantly （P〈0.01）, and the cell proliferation depressed rate showed the dependent relation to the dosage. The observation of light microscope and AO/EB fluorescent staining displayed that cells in the experimental group partly showed typical apoptosis. The morphology of cells was changed such as nuclear chromosomal condensation and segmentation. Agarose gel electrophoresis analysis showed DNA cleavages（DNA ladder）. The Caspase-3 activity significantly increased （P 〈0. 05） comparing with the control cells, and the activity of enzyme showed the dependent relation to the dosage. Conclusion. UA induces the apoptosis, and the increasment of Caspase-3 may contribute to its effects.

关 键 词：熊果酸 细胞增殖 凋亡 活性半胱天冬酶-3 

分 类 号：R996[医药卫生—毒理学]