无菌培养条件下不同基因型小麦耐盐性及其生理机制研究  

Study on salt tolerance of different winter wheat genotypes and their physiological mechanism under sterile condition

作　　者：王俪梅[1] 李青松[1] 周春菊[2] 尚浩博[1] 王林权[1] 

机构地区：[1]西北农林科技大学资源环境学院,陕西杨凌712100 [2]西北农林科技大学生命科学学院,陕西杨凌712100

出　　处：《干旱地区农业研究》2009年第4期99-104,共6页Agricultural Research in the Arid Areas

基　　金：国家自然科学基金项目(30571085)

摘　　要：以4种不同基因型小麦（小偃6号、NR9405、RB6和陕229）种子为材料,研究组培条件下盐胁迫对小麦种子活力、幼苗生物量、保护酶活性、亲合性渗调物质以及Na＋、K＋含量等的影响。结果表明：盐胁迫下,小麦幼苗的生长均受到不同程度的抑制,表现为发芽率、根长、芽长、生物量随着盐浓度的升高而明显下降,并存在显著的基因型差异。盐胁迫下小偃6号与NR9405的生物量显著高于陕229与RB6;小偃6号与NR9405的超氧化物歧化酶（SOD）活性均随着盐浓度的增大而升高;RB6和陕229在0-150 mmol/L盐浓度下SOD酶活性升高,而在200mmol/L盐浓度时,SOD活性急剧下降。随着盐浓度的升高4种不同基因型小麦体内游离脯氨酸累积量增大,在200mmol/L时RB6的累积量显著高于其它基因型。随着盐浓度的升高,幼苗体内的Na＋含量增加,K＋含量下降,Na＋/K＋比值增大。小偃6号和NR9405的耐盐性优于陕229与RB6。The seed vigor, seedling biomass, protective enzyme activity and compatible osmolytes as well as Na^＋ and K^＋ content of four wheat genotypes seedling under salt stress were studied in vitro. The results indicated that the seed germination and seedling growth were inhibited under the salt stress. The germination rate, single root length, bud length and biomass decreased significantly as the increase of salt concentration, and there were significant differences among genotypes. The biomass of Xiaoyan 6 and NR9405 were significantly greater than Shaan229 and RB6 under salt stress. SOD activity of Xiaoyan and NR9405 increased with the increase of salt concentration. SOD activity of RB6 and Shaan229 increased under the salt concentration of 0 -150 mmol/L; however, it decreased sharply under the 200 mmol/L salt concentration. As the increase of salt concentration, the free proline content in four different wheat genotypes increased, and the accumulation in RB6 was significantly higher than others under the 200 mmoL/L salt concentration. The Na^＋ content and Na^＋/K^＋ in wheat seedlings rose but K^＋ content dropped owing to the increase of salt concentration. Overall, Xiaoyan6 and NR9405 were better than Shaan229 and RB6 in salt tolerance.

关 键 词：盐胁迫 小麦 SOD 游离脯氨酸 

分 类 号：S512.1[农业科学—作物学]