祛瘀生新法对药物流产后大鼠子宫蜕膜细胞凋亡影响的实验研究  

Lnves Tigate Function Mechanism of Quyu Shengxin How to Redule Hemorvhage After Medical Aloortion of Rats

作　　者：于燕[1] 张燕[2] 周景华[1] 王晶[3] 金富锐[4] 吴效科[1] 侯丽辉[1] 

机构地区：[1]黑龙江中医药大学附属医院,哈尔滨150040 [2]黑龙江中医药大学,2006级研究生哈尔滨150040 [3]哈尔滨医科大学第三附属医院,150081 [4]大连大学附属中山医院,116001

出　　处：《光明中医》2009年第8期1469-1471,共3页GUANGMING JOURNAL OF CHINESE MEDICINE

基　　金：黑龙江省自然科学基金(No:D200649);黑龙江省教育厅科研项目(No:11521264);哈而滨市科技创新人才培养专项资金项目(No:2006RFQXS106)

摘　　要：目的:探讨祛瘀生新代表方剂生化止血饮减少药物流产后阴道出血的作用机制。方法:建立正常对照组,模型组,益母草组,生化止血组,采用原位末端标记法(TUNEL)检测凋亡的发生及凋亡细胞的面密度;采用免疫组化法检测大鼠子宫蜕膜中凋亡基因Fas、Fasl变化。结果:祛瘀生新代表方剂生化止血饮可显著增加子宫蜕膜中凋亡细胞的面密度,增强加Fas、Fasl的表达水平(P<0.01),与益母草组和药流模型组比较也有显著性差异(P<0.05),生化止血饮的疗效优于益母草组及模型组。结论:祛瘀生新代表方剂生化止血饮能通过增强凋亡细胞的面密度,增强蜕膜中Fas、FasL的表达水平促进蜕膜剥脱加速,缩短药流后阴道出血的时间,达到减少阴道出血量的目的。Objective： To investigate the function mechanism of Shenghuazhixueyin which is the representative of Quyushengxin how to reduce the hemorrhage after the medical abortion. Method：establish the normal group, the model group, yimucao group, shenghuazhixueyin group, then detect the apoptosis＇ happen and the apoptosis cell＇s areal density by the way of TUNEL, and detect the apoptosis gene Fas, Fasl＇ s change in rat＇s womb decidua by the immunohistochemical method. Result： Shenghuazhixueyin can remarkably increase the apoptosis cell＇s areal density in womb decidna improve the expression of Fas,Fasl（ P 〈 0.01 ）. Comparing with yimucao group and the medical abortion group there are significant differences（ P 〈 0.05）. Shenghuazhixueyin group has a better curative effect than yimucao group and the medical abortion group. Conclusion： Shenghuazhixueyin can increase the apoptosis cell＇s areal density and improve the expression of Fas,Fasl. Through that way, it will make residual decidua flake quickly, reduce the colporrhagia time and reach the purpose of hematischesis.

关 键 词：祛瘀生新 药流后出血 细胞凋亡 

分 类 号：R285.5[医药卫生—中药学] R169.4[医药卫生—中医学]