阵列细胞微培养腔的设计与制造  

Design and Fabrication of Array Based Cell Culture Chamber

作　　者：张瑞强[1,2] 杨军[1] 金庆辉[2] 赵建龙[2] 郑小林[1] 

机构地区：[1]重庆大学生物工程学院,重庆400030 [2]中国科学院上海微系统与信息技术研究所,上海200050

出　　处：《传感技术学报》2009年第8期1071-1076,共6页Chinese Journal of Sensors and Actuators

基　　金：国家自然科学基金资助(30771115;30770569;30870661);973项目资助(2007CB714502;2007CB936000);上海市科委项目资助(0752nm021;0852nm00400)

摘　　要：采用光刻成型技术、"多次光刻、一次显影"工艺以及热键合工艺制备了基于三层、具有阀控开关和浓度梯度的PDMS微阵列细胞微培养腔,重点研究了浓度梯度、细胞培养腔结构的设计。研究表明浓度梯度的形成主要受芯片管道结构影响;在低雷诺数、被动混合方式情况下,采用简单结构进行混合的效率会很低;在本课题中,将瓦片状结构与管道复合起来提高混合效率,实验结果表明:即使在高流速情况下,也能得很高的混合效率并使各管道间浓度呈很好的线性分布;同时,对细胞培养腔的结构进行了设计;此外,将有限元分析软件COMSOL应用到混合管道及培养腔内流体、流态进行了分析,进一步利用分析结果优化了结构设计。The fabrication of the amicro chamber array for cell culture, Array based on micro cell culture chamber was successfully completed by Polymer-MEMS technology, employing three SU-8 layers without development after exposure until the end of the process and the micro cell culture chip was equipped with valves utilized to switch switched the channels for cell infusion on and off and could generate different concentrations. In this paper, formation of concentration and design of structures of cell culture chamber were mainly discussed. It was shown concentration contour was mostly influenced by channel structures;however, due to passive mixing, the mixing efficiency was quite low under low Reynolds, so tile-like structure was chosen to improve the mixing efficiency. It was demonstrated in the experiment that this kind channel be perfect and the concentration in the end of each channel from this side to the other was distributed linearly. Meanwhile,we also did some improvements on the cell culture chamber. What＇s more, the finite element software COMSOL was utilized in simulation and the output was also used for the design of structure.

关 键 词：微流控 培养腔 COMSOL PDMS 

分 类 号：TN305.7[电子电信—物理电子学]