不同产地金银花药材高效液相指纹图谱的研究  

HPLC fingerprints of Lonicera japonica Thunb from different places

作　　者：杨雪萍[1] 袁红英[1] 李峰[2] 

机构地区：[1]中国人民解放军第88医院药剂科,泰安271000 [2]山东中医药大学,济南260014

出　　处：《江西中医学院学报》2009年第4期38-40,共3页Journal of Jiangxi College of Traditional Chinese Medicine

摘　　要：目的:研究并建立三个不同产地金银花药材的指纹图谱。方法:采用岛津C18色谱柱(250 mm×4.6 mm,5μm);以乙腈-0.1%磷酸水溶液梯度洗脱,流速1.0 ml/min,柱温40℃,检测波长254 nm。结果:建立了金银花药材HPLC指纹图谱共有模式,三个不同产地金银花相似度均在0.95以上。结论:该方法特征性强、简便。Objective ：To study and establish the fingerprints of Lonicera japonica Thunb from different places by HPLC. Methods：The fingerprints of Lonicera japonica Thunb was built by using Diamonsil C18 as analytical column and acetonitrile-0. 05 H3PO4 aqueous in gradient as a mobile phase. The flow rate was 1.0 ml/min. Detecting wavelength was set at 254nm. The temperature of column was at 40 ℃. Results：The mutual mode to HPLC fingerprints was set up, the similarities in 3 batches of Lonicera japonica Thunb samples were all above 0.95. Conclusion ：This methods is simple, stable and reproducible.

关 键 词：不同产地 金银花 HPLC 指纹图谱 

分 类 号：R284.1[医药卫生—中药学]