大孔树脂对凤尾草水部位总黄酮的富集作用研究  

Study on Accumulation of the Total Flavonoids of Water Soluble Fraction in Pteris multifida by Macroporous Resin

作　　者：刘建群[1] 张维[1] 张锐[1] 李青[1] 赵元[1] 

机构地区：[1]江西中医学院现代中药制剂教育部重点实验室,南昌330004

出　　处：《江西中医学院学报》2009年第4期49-51,共3页Journal of Jiangxi College of Traditional Chinese Medicine

基　　金：江西省卫生厅中医药科研计划项目(2008B007)

摘　　要：目的：研究风尾草水部位中总黄酮含量经大孔树脂吸附前后的变化情况，建立凤尾草水部位中总黄酮含量测定的方法．为其提取工艺优化、化学成分研究及质量评价提供依据。方法：凤尾草采用70％乙醇提取，分部位萃取后，得水部位，利用LSA-40大孔树脂对凤尾草中水部位的总黄酮进行富集，采用NaNO2-Al（NO3）3-NaOH显色，利用紫外分光光度法测定凤尾草中的总黄酮含量，检测波长为510nm。结果：回归方程为C=0．0871A＋0．0007，R=0．9998，总黄酮质量浓度在0．004～0．04mg／ml范围内与吸收度线性关系良好，水部位以及大孔树脂95％乙醇洗脱部位平均加样回收率分别为99．64％和99．99％，RSD为1．16％和1．47％。测得凤尾草水部位总黄酮含量为5．16％，经LSA-40大孔树脂富集后，总黄酮含量为27．08％，黄酮含量提高了5倍多。结论：该凤尾草水部位总黄酮测定方法操作简便，稳定性好，结果可靠；LSA-40大孔树脂对风尾草水部位总黄酮有很好的富集作用。Objective：To study the accumulation of the total flavonoids of the water soluble fraction in Pteris multifida by macroporous resin. Methods：The total flavonoids of the water soluble fraction were accumulated by the macroporous resin（LSA-40） and determined by UV spectrophotometry at wavelength 510 nm by NaNO2-Al （NO） 3-NaOH ehromogenic agent. Resuhs：A UV spectrophotometry for the determination of the total flavonoids of the water soluble fraction in Pteris multifida was developed. Under the selected eperimental conditions, the regression equation was C = 0. 087 1A ＋ 0. 000 7, r = 0.999 8, the linear range was 0. 004 - 0.04 mg/ml. The average recoverys were 99.64% （ RSD 1.16% , n = 6 ） and 99.99% （ RSD 1.47% , n = 6 ） for the samples before and after treated by macroporous resin（ LSA-40 ）. The total flavonoid contents of the water soluble fraction in Pteris multifida were increased from 5.16% to 27.08% after treated by maeroporous resin（LSA-40）. Conclusion ：The UV spectrophotometry was simple, stable and reliable for the determination of the total flavonoids of the water soluble fraction in Pteris multifida. The total flavonoids of the water soluble fraction in Pteris multifidacan be accumulated by macroporous resin（LSA-40） with excellent efficiency.

关 键 词：凤尾草 总黄酮 紫外分光光度法 含量测定 大孔树脂 

分 类 号：O657.32[理学—分析化学]