生殖道衣原体感染的临床疗效观察指标的探讨  

Investigation on indicators of clinical therapeutic effect of choamydiae infection in the gential tract

作　　者：邓群[1] 卢培恩 

机构地区：[1]湖北省宜昌市第一人民医院检验科,443000 [2]浙江省宁波市检验检疫局保健中心,315012

出　　处：《国际检验医学杂志》2009年第8期746-747,共2页International Journal of Laboratory Medicine

摘　　要：目的通过实验探讨生殖道沙眼衣原体感染临床疗效观察的指标。方法用McCoy细胞分离培养、实时荧光定量PCR和一步免疫层析法对首诊单纯衣原体阳性的患者同时进行临床疗效的监测。结果治疗一周后,细胞培养法和荧光定量PCR检测法转阴率分别为53.6%、51.8%,而一步免疫层析法仅为5.4%。三周后细胞培养法达96.4%;四周后荧光定量PCR达94.6%;五周后一步免疫层析法达96.4%。结论荧光定量PCR和一步免疫层析法均不能成为短期疗效观察的指标。Objective To investigate the indicators of clinical therapeutic effect of choarnydiae infection in the gential tract by experiments. Methods McCoy cell isolation and culture method, real-time fluorescence quantitative PCR（RT-PCR） and step immunoehromatographic method were applied to monitoring the clinical therapeutic effect of the first-diagnosed patients with single choarnydiae infection. Results After one week＇s treatment, the negative turnover rates were as follows：53.6 % （cell culture assay） ; RT-PCR（51.8%） ; 5.4 % （ step immunochromatographic method）. Three weeks later, the negative turnover rates of cell culture assay accounted for 96.4% ;four weeks later, the negative turnover rates of RT-PCR accounted for 94.6% ;while five weeks later,the negative turnover rates of step immunochromatographic method accounted for 96.4%. Conclusion Both RT-PCR and step immunochromatographic method can not be used as means of short-term therapeutic effect evaluation.

关 键 词：衣原体科感染 泌尿生殖系统 微生物敏感性试验 

分 类 号：R711.3[医药卫生—妇产科学] R446.5[医药卫生—临床医学] R450