家鸭×番鸭杂种下调表达新基因r-MHD2的克隆  

Cloning of a Hybrid Down-regulated Gene r-MHD2 in Domestic Duck and Muscovy Duck Crossbreeding System

作　　者：单雪松[1] 陈洋[1] 崔庆媛[1] 李克鹏[1] 

机构地区：[1]吉林农业大学动物科学技术学院,吉林长春130118

出　　处：《家畜生态学报》2009年第4期14-17,共4页Journal of Domestic Animal Ecology

基　　金：国家自然科学基金项目(30700570)

摘　　要：以北京鸭、法国番鸭及其正反交F1代杂种为试验材料,采用mRNA差异显示(DDRT-PCR)方法分析不同试验组肌肉组织基因表达差异,结合抑制性消减杂交(SSH)加以验证,发现一个杂种下调表达基因r-MHD2,对鸭r-MHD2基因698 bp片段BLASTING结果表明,该基因与鸡(Gallus gallus)、非洲爪蟾Xenopus laevis和Xenopus tropicalis同源性分别为91%、78%和77%。r-MHD2基因的具体分子功能尚属未知。The gene expression of muscle tissue from Beijing duck, France muscovy duck and their mutual hybrids were examined with the method of DDRT-PCT, and the display result was verified by the method of SSH. One hybrid down-regulated gene r-MHD2 was found in this research. The sequencing and blasting result of the 698bp fragment of duck r-MHD2 gene showed that the identity of this gene and Gallus gallus, Xenopus laevis and Xenopus tropicalisgene is 91%, 78% and 77% respectively.

关 键 词：MHD2基因 杂种优势 MRNA差异显示 鸭 番鸭 

分 类 号：S811.5[农业科学—畜牧学]