玉米矮花叶病抗病基因Rscmv1新型标记的开发  

Development of Novel Markers of the Resistance Gene Rscmv 1 to Maize Dwarf Mosaic

作　　者：王永霞[1] 赵荣兵[1] 丁俊强[1] 张学才[1] 陈甲法[1] 武轲[2] 吴建宇[1] 

机构地区：[1]河南农业大学农学院,河南郑州450002 [2]河南农业大学生命科学学院,河南郑州450002

出　　处：《河南农业科学》2009年第8期84-87,共4页Journal of Henan Agricultural Sciences

基　　金：国家高技术研究863计划项目(2006AA10Z1D5);河南省高等学校创新人才培养对象(豫教高[2004]294号)

摘　　要：在玉米矮花叶病抗病基因Rscmv 1初步定位的基础上,利用生物信息学方法在抗病区域内进行BAC-SSR和内含子长度多态性标记(ILP)2种新型标记的开发。共找到16个在抗病材料四一和感病材料Mo17中有多态性的标记,其中BAC-SSR标记12个,ILP标记4个,为抗病基因Rscmv 1的精细定位和图位克隆奠定了基础。Two new types of markers BAC - SSR and ILP respectively were developed by bioinfor- matics in fragment of resistance gene on the basis of preliminary location of the resistance gene Rscmv 1. Total 16 markers exhibited polymorphisms between Siyi and Mo17, including 12 BAC- SSR makers and 4 ILP makers. Which laid the foundation for fine mapping and map-based cloning of the resistance gene.

关 键 词：基因定位 BAC—SSR标记 内含子长度多态性标记 玉米矮花叶病 

分 类 号：S513.034[农业科学—作物学]