鼠源性神经生长因子对缺氧缺血性脑损伤大鼠海马胶质纤维酸性蛋白表达的影响  

Influence of mouse nerve growth factor on glial fibriUary acidic protein expression in hippocampus of neonatal rats with hypoxic-ischemic brain damage

作　　者：尹晓娟[1] 刘冬云[1] 罗分平[1] 龙琦[1] 封志纯[1] 

机构地区：[1]北京军区总医院附属八一儿童医院新生儿科,100700

出　　处：《中国小儿急救医学》2009年第4期352-354,I0001,共4页Chinese Pediatric Emergency Medicine

基　　金：基金项目：中国博士后基金（20070410505）

摘　　要：目的 探讨鼠源性神经生长因子（mNGF）对HIBD新生大鼠海马胶质纤维酸性蛋白（GFAP）表达的影响。方法新生7日龄SD大鼠60只，随机分为mNGF干预组（／1=20）、HIBD组（／1：20）、正常对照组（／1=20）。mNGF干预组在HIBD模型建立后予以mNGF20ng／（g·d）臀部肌肉注射，连用5d。HIBD组未予处置。正常对照组予0．1mol／LPBS10μl（g·d）臀部肌肉注射，连用5d。3组大鼠在实验开始后7、14d各处死10只。采用免疫组织化学技术检测3组大鼠海马组织GFAP表达的动态变化。结果在7d时，mNGF干预组、HIBD组GFAP在病侧海马的表达均较正常对照组增加，但mNGF干预组GFAP主要表达在病侧海马的DG区，HIBD组GFAP主要表达在病侧海马的CA1区。在14d时，HIBD组GFAP在病侧海马的表达减少；mNGF干预组GFAP在病侧海马的表达进一步增加，且较正常对照组增加。结论mNGF能增加HIBD大鼠海马GFAP的表达，具有神经修复作用。Objective To investigate the influence of mouse nerve growth factor （mNGF）on glial fibrillary acidic protein （GFAP） expression in hippocampus of neonatal rats with hypoxic-ischemic brain damage （HIBD）. Methods Sixty neonatal rats of 7 days old were randomly divided into control group （n =20）,HIBD group （n = 20）and mNGF group （n = 20）. After HIBD model established, mNGF of 20 ng/（ g·d） was given by intramuscular injection in buttocks in mNGF group, continued five days. No intervention was given in HIBD group. 0. 1 mol/L PBS of 10 μl/（g·d） was given by intramuscular injection in buttocks for the control group,continued five days. Ten rats were sacrifled at 7 d and 14 d after intervention respectively in three groups. The expressions of GFAP in hippocampus were determined with immunohistochemical method. Results At 7 d and 14 d after intervention, the expression levels of GFAP in sick side hippocampous in HIBD group and mNGF group were higher than that in control group. GFAP positive cell mainly distributed in sick side hippocampal DG region in mNGF group, while CA1 region in HIBD group. As compared with 7 d, the expression of GFAP in sick side hippocampus in HIBD group decreased at 14d, while increased in mNGF group. Conclusion mNGF can increase the expression of GFAP in hippocampus of HIBD neonatal rat, and mNGF may have nervous repairing role to HIBD.

关 键 词：缺氧缺血性脑损伤 神经生长因子 胶质纤维酸性蛋白 新生鼠 

分 类 号：R722.1[医药卫生—儿科]