重组质粒pZeoSV2(+)/CpG-HBcAg(ISS)对BALB/c小鼠的免疫作用  

Immune Effects of Recombinant Plasmid pZeoSV2(+)/CpG-HBcAg(ISS) on BALB/c Mouse

作　　者：吴会玲[1] 田德英[1] 周健[1] 许东[1] 陈淼[1] 章述军[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院,武汉430030

出　　处：《内科急危重症杂志》2009年第4期193-196,共4页Journal of Critical Care In Internal Medicine

基　　金：湖北省自然科学基金资助项目(No:2006ABA139)

摘　　要：目的:探讨重组质粒pZeoSV2(+)/CpG-HBcAg(ISS)对BALB/c小鼠免疫的作用。方法:构建真核表达质粒pZeoSV2(+)/CpG-HBcAg(ISSb,c),将其免疫BALB/c小鼠,ELISA法检测免疫后小鼠血清中IFN-γ、IL-2、IL-12、IL-4和IL-10的含量。结果:pZeoSV2(+)/CpG-HBcAg(ISSb)组较pZeoSV2(+)/CpG-HBcAg(ISSc)组免疫BALB/c小鼠后能使Th1型细胞因子IFN-γ、IL-2和IL-12的表达增强,抑制Th2型细胞因子IL-4和IL-10的产生。结论:含CpG-HBcAg(ISSb)的重组质粒能够明显诱导小鼠血清Th1型细胞因子的表达,抑制Th2型细胞因子的表达。Objective： To explore the immone effects of recombinant plasmid pZeoSV2（ ＋ ）/CpG-HBcAg（ISS） on BALB/c mice. Methods： Recombinant plasmid pZeoSV2（ ＋ ）/CpG-HBcAg（ISSb, c） was constructed and was used to immune BALB/c mice. The serum IFN-γ, IL2, IL-12, IL-4 and IL-10 level were detected by ELISA. Resuits： The IFN-γ, IL2 and IL-12 induced by pZeoSV2 （ ＋ ）/CpG-HBcAg （ISSb）group were obviously higher than those of pZeoSV2 （＋）/CpG-HBcAg（ISSc）group （P（0. 01）, as well as , levels of IL-4 and IL-10 induced by pZeoSV2（ ＋ ）/CpG-HBcAg（ISSb）group were obviously less than those of pZeoSV2（ ＋ ）/CpG-HBcAg（ISSc）group （P〈0. 01）. Conclusions： The result showed that high levels of Thl type cytokines including IL-2, IFN-γ and IL-12 were induced in pZeoSV2（ ＋ ）/CpG-HBcAg（ISSb）recombinant plasmid groups, where as the levels of Th2 type cytokines such as IL-4 and IL-10 were inhibited obviously.

关 键 词：CPG基序 乙肝核心抗原 Th1/Th2 型细胞因子 

分 类 号：R512.6[医药卫生—内科学]