都匀龙胆草的组织培养与快速繁殖技术  

Tissue Culture and Rapid Propagation of Gentian seabra in Duyun City

作　　者：郭治友[1] 刘春芳[1] 罗应[1] 钱绍方[1] 

机构地区：[1]黔南民族师范学院生命科学系民族生物资源研究,贵州都匀558000

出　　处：《贵州农业科学》2009年第8期31-33,共3页Guizhou Agricultural Sciences

基　　金：黔南民族师范学院2007年科研机构专项"黔南民族生态旅游资源调查与开发研究"

摘　　要：为较系统地建立地道大宗药材都匀龙胆草(Gentianaseabra Bunge)的无性快繁体系,实现其中药产业现代化大量的种苗需求,对都匀龙胆草的组织培养与快速繁殖进行了试验研究。结果表明:以MS+6-BA 1.0 mg/L+IBA 3.0 mg/L为培养基较利于愈伤组织诱导;以MS+6-BA 1.1 mg/L+IBA 1.0 mg/L为培养基较利于丛生芽的增殖,增殖系数达8.6;以MS+6-BA 0.5 mg/L+NAA 0.2 mg/L为培养基较有利于生根培养。首次报道培养基中添加AC 3.0 g/L可以有效控制龙胆草组织培养过程中出现的褐化现象。The in vitro rapid propagation technique, callus induction of different explants, multiplication of cluster buds and rooting induction of Gentiana Seabra were tested to set up its asexual rapid propagation system for providing a great quantity of seedlings. The results showed that the optimum medium for callus induction, cluster bud multiplication and rooting induction is MS＋6-BA 1.0 mg/L＋IBA 3.0 mg/L, MS＋6-BA 1.1 mg/L ＋IBA 1.0 mg/L and MS＋6-BA 0. 5 mg/L＋NAA 0. 2 mg/L respectively and the browning phenomenon in tissue culture of Gentiana Seabra can be effectively controlled by adding AC 3.0 g/L in the medium.

关 键 词：龙胆草 组织培养 离体快速繁殖 

分 类 号：S567.9[农业科学—中草药栽培]