固相萃取反向冲洗-反相HPLC法测定血浆中醋氯芬酸浓度  

Determination of Aceclofenac in Plasma by RP-HPLC with Solid Phase-Back Flushing Extaction Method

作　　者：高立勤[1] 刘文英[1] 邢久东[1] 

机构地区：[1]中国药科大学

出　　处：《药物分析杂志》1998年第4期219-222,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：建立了反相ＨＰＬＣ法，测定血浆中醋氯芬酸的浓度，并对单剂量口服给药后的药代动力学进行了初步的探讨。血浆样本经大孔树脂小柱萃取后，以ＯＤＳ柱分析，流动相为甲醇—水—冰醋酸（６２∶３６∶２），检测波长为２７５ｎｍ。在０５～１００μｇ／ｍＬ浓度范围内线性关系良好，最低检出限为０３μｇ／ｍＬ。A RP-HPLC method has been developed to determine the concentration of aceclofenac in plasma.After extracted by a macroreticular resin cartridge with back-flushing method,the plasma sample was analysed on an ODS column.The mobile phase is methanol-water-glacial acetic acid(62∶36∶2).The detective wavelength is 275 nm.In the concentration range of 05～100 μg/mL,a good linearity can be found.The inter-day and intra-day precision are all less than 10 %.The method was used in the pharmacokinetic study of aceclofenac.The results from three volunteers shew that the drug acted more likely in two compartment model.The plasma concentration got peak value at 3 h after the admininstration.And the t1/2(β) value of 324 h revealed that the elimination speed of the drug is a little bit slow.

关 键 词：固相萃取 反向冲洗 HPLC 醋氯芬酸 含量测定 

分 类 号：R971.1[医药卫生—药品] R927.2[医药卫生—药学]