反相高效液相色谱法测定银翘解毒片中绿原酸和甘草酸的含量  

The Studies on Separation and Determination of Chlorogenic Acid and Glycyrrhetic Acid in Yin Qiao Jie Du Tablets by RP-HPLC

作　　者：倪坤仪[1,2] 韩南银[1,2] 屠洁 

机构地区：[1]中国药科大学分析化学研究室 [2]深圳市医药研究设计所

出　　处：《药物分析杂志》1998年第4期252-255,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：用反相高效液相色谱法测定了银翘解毒片中绿原酸和甘草酸，此２种成分分别为该成药中主药金银花和甘草的有效成分。用十八烷基硅烷微粒硅胶为固定相，乙腈—水（４９∶５１）为流动相，非那西丁为内标，紫外检测。用溶剂选择性三角形对流动相进行了优化，对提取溶剂、提取方法、干扰因素进行了考察，并进行了线性范围、模拟样回收率和加样回收率试验。测定了３个批号的样品。该法也可用于金银花、甘草原药材中绿原酸、甘草酸的含量测定。In this paper a RP-HPLC method was developed to separate and determine chlorogenic acid and glycyrrhetic acid in Yin Qiao Jie Du tablets.They were separated and determined on YQG C18 column(39 mm×200 mm,5μm),using acetonitrile-water(49∶51)as mobile phase,phenacetin as internal standard,UV detector,flow rate 1 mL/min.Selection of extracting solvent,determination of linearity range and check of interference factors have been made.The accuracy of the method was checked by recoveries.

关 键 词：银翘解毒片 绿原酸 甘草酸 反效液相色谱 

分 类 号：R286.0[医药卫生—中药学]