器官移植术后的嵌合现象  

Study on organ transplantation chimerism by polymerase chain reaction technique

作　　者：江志伟[1] 黎介寿[1] 李宁[1] 尹路[1] 李幼生[1] 

机构地区：[1]南京军区南京总医院全军普通外科研究所

出　　处：《中华器官移植杂志》1998年第3期149-150,共2页Chinese Journal of Organ Transplantation

基　　金：国家自然科学基金

摘　　要：为研究器官移植术后的嵌合现象，术后利用聚合酶链反应（ＰＣＲ）技术检测３例接受男性供体器官的女性受者外周血及皮肤组织中的Ｙ染色体特异性ＤＮＡ片段。结果在１例小肠移植受者的外周血及皮肤组织中出现Ｙ染色体特异性ＤＮＡ片段；２例肾移植受者的外周血中也出现Ｙ染色体特异性ＤＮＡ片段。表明在器官移植术后存在着供体细胞向受体组织的移行嵌合。认为促进嵌合的出现及保持嵌合的平衡，将有利于防治排斥反应和移植物抗宿主病。In order to investigate the organ transplantation chimerism, 3 female patients receiving male donor grafts were selected to detect a Y chromosome specific DNA fragment to further identify the presence of donor cells based on sex difference between donor and recipient by using polymerase chain reaction (PCR). Y chromosome specific DNA fragment are found in peripheral blood and skin of one recipient undergoing small bowel transplantation, and in peripheral blood of 2 recipients undergoing renal transplantation. The results indicated that the donor cells migrated to the recipients’ tissue. It was suggested that chimerism and the maintainance of chimerism balance would be of benefit to the prevention and treatment of rejection and graft versus host disease.

关 键 词：移植物生存 器官移植 嵌合现象 

分 类 号：R617[医药卫生—外科学]