可乐定对中脑导水管周围灰质脑啡肽和P物质释放的影响  

Effects of clonidine on releases of enkephalin and substance P from periaqueductal gray

作　　者：王红兵[1] 金士翱[2] 茹立强[1] 

机构地区：[1]同济医科大学附属协和医院麻醉科,武汉市430022 [2]同济医科大学附属同济医院麻醉科,武汉市430022

出　　处：《中华麻醉学杂志》1998年第7期422-423,共2页Chinese Journal of Anesthesiology

摘　　要：目的:通过观察可乐定对大鼠中脑导水管周围灰质脑啡肽和P物质释放的影响,来探讨中枢α_2受体激活后参与镇痛的神经递质成分。方法:20只大白鼠随机分为可乐定组和对照组,各10只。可乐定组大鼠腹腔注射可乐定40μg,对照组注射等量生理盐水。30分钟后,多聚甲醛固定中脑,冰冻切片。脑啡肽和P物质免疫组化显示用过氧化物酶-抗过氧化物酶法(PAP法)。结果:与对照组相比,可乐定处理的大鼠中脑导水管周围灰质的脑啡肽免疫反应产物显著减少(P<0.05),P物质免疫反应产物无明显变化(P>0.05)。结论:可乐定通过兴奋中枢α_2受体,促进中脑导水管周围灰质释放脑啡肽起到镇痛作用。可乐定镇痛与中脑P物质无关。Objective:The stimulative effects of clonidine on release of enkephalin and substance P from periaqueductal gray was studied to analyse the analgesic neurotransmitters related to alpha 2 adrenoceptor activation. Method: Twenty SD rats were allocated in pairs to receiving intraperitoneal clonidine 40μg in clonidine group or normal saline in control group. Immunohistochemical technique was used to measure the contents of enkephalin and substance P in slice of rat midbrain. Result:The immunoreaction of enkephalin from slice of rat midbrain was decreased significantly in clonidine group compared with control group(P<0.05), and the contents of substance P was no different between two groups. Conclusion:The analgesia effect of intraperitoneal clonidine results from the release of enkephalin by the activation of the central alpha 2-adrenoceptor,but is not related to substance P in periaqueductal gray.

关 键 词：可乐定 脑啡肽 导水管周灰质 

分 类 号：R972.4[医药卫生—药品] R338.3[医药卫生—药学]