SRB法和MTT法抗肿瘤药物筛选结果相关性研究  

Correlation between experimental results of MTT colorimetric assay and SRB assay in anti-tumor drug screening in vitro

作　　者：黄银久[1,2] 宋宝安[2] 金林红[2] 胡德禹[2] 杨松[2] 李宁[2] 吴守伟[1] 

机构地区：[1]蚌埠医学院生物科学系,蚌埠233000 [2]贵州大学精细化工研究开发中心,“绿色农药与生物工程”教育部重点实验室,贵阳550025

出　　处：《生物学杂志》2009年第4期13-16,共4页Journal of Biology

基　　金：国家973计划(2003CB114404);科技部重点项目计划(2005DF100660);安徽省教育厅自然科学研究项目(2006KJ124C)

摘　　要：为了探索抗肿瘤药物体外筛选中磺酰罗丹明B(SRB)法和四甲基偶氮唑盐(MTT)比色法实验结果的相互验证作用,作者以PC-3、BGC-823、Bcap-37细胞系为研究对象,利用SRB法和MTT比色法对供试化合物行体外抑制活性考察,并用SPSS12.0对两种筛选方法得到的实验结果进行差异性分析。结果表明,SRB法和MTT比色法实验结果相关性好,可以相互验证实验结果的准确性和可靠性。In order to investigate the correlation between experimental results of MTT colorimetric assay and SRB assay in antitumor drug screening in vitro, inhibition activity of ADM, pentadienone Compound 2005244 and quinazoline compounds 2007238, 2007281, 2007283, 2007284 against PC-3, BGC-823 and Bcap-37 cell lines were tested respectively. The experimental data were made with significant analysis by SPSSI2.0 in oneway Anova process. It was found the correlation between experimental results of MTT colorimetric assay and SRB assay was good. The two methods are accuracy and probability.

关 键 词：MTT比色法 SRB法 相关性 

分 类 号：R965.1[医药卫生—药理学]