肝脏部分切除术对不同月龄小鼠皮质酮分泌和学习记忆功能的影响  

Effects of partial hepatectomy on serum corticosterone level and learning and memorial function in mice of different ages

作　　者：鹉日歌牧乐 王东信[1] 周岩[1] 

机构地区：[1]北京大学第一医院麻醉科,北京100034

出　　处：《武警医学》2009年第8期674-678,共5页Medical Journal of the Chinese People's Armed Police Force

基　　金：国家自然科学基金(30740050)

摘　　要：目的观察肝脏部分切除术对不同月龄小鼠皮质酮分泌和学习记忆功能的影响。方法分别将4月龄和16月龄C57BL/6J小鼠随机分为3个亚组,即对照组、麻醉组、手术组。经相应处理后12 h每组随机抽取10～15只小鼠测定血清皮质酮浓度,其余各组小鼠于第31天进行Morris水迷宫测试。结果(1)血清皮质酮浓度:在同月龄小鼠的3个亚组中,手术组明显高于对照组和麻醉组(P<0.05或P<0.01),但麻醉组与对照组之间比较差异无统计学意义。在不同月龄小鼠间,16月龄小鼠的对照组和麻醉组均明显高于4月龄小鼠的相应各组(P<0.05或P<0.01);(2)水迷宫测试潜伏期:4月龄组小鼠在各时间点3个亚组间均无明显差异。而16月龄组小鼠的手术组在第1、2、4、5天时均明显短于对照组(P<0.05或P<0.01);(3)水迷宫测试总路程:4月龄小鼠手术组在第1天时明显长于对照组和麻醉组(P均<0.05)。16月龄小鼠手术组在第2、4、5天时明显短于对照组(P<0.05或P<0.01);麻醉组在第2天时明显短于对照组(P<0.05)。结论肝脏部分切除术对两种月龄小鼠均造成了明显的应激反应。该反应对4月龄小鼠术后远期学习记忆功能无明显影响,但改善了16月龄小鼠术后远期的学习记忆功能。Objective To study the effects of partial hepatectomy on serum corticosterone level and learning and memorial function in mice of different ages. Methods One hundred and seventy - seven healthy male C57BI/6J mice, of which 86 were 4 months old and 90 were 16 months old, were randomly divided into three groups：control group, anesthesia group,and surgery group. Twelve hours later, 10 to 15 mice were randomly selected from each group and blood samples were taken for measurement of serum cor- ticosterone level. Morris water maze testing was performed from the 31st day. Results （ 1 ） Among the 3 subgroups of the same age mice, the serum corticosterone levels in the surgery groups were significantly higher than those the control and the anesthesia groups （P 〈 0.05 or P 〈 0.01 ）. When compared between the mice of different ages ,the steroid levels in the control and the anesthesia groups of 16 month - old mice were both significantly higher than those in the corresponding groups of 4 month - old mice （P 〈 0.05 or P 〈 0.01 ）. （2） Latency period of Morris testing. In 4 month - old mice, no significant differences existed among the 3 subgroups in each time points. In 16 month- old mice,the periods in the surgery group were significantly shorter than those in the control group on the 1st, 2nd, 4th, and 5th training days （P 〈0.05 or P 〈0. O1 ）, and were significantly shorter than those in the anesthesia group on the 3rd, 4th, and 5th training days （P 〈0.05 or P 〈0.01）. （3） Swimming distances of Morris task. In 4 month - old mice, the distance in the surgery group was significantly longer than those in the control and the anesthesia groups on the 1st training day （P 〈0.05）. In 16 month- old mice,the distance in the surgery group was significantly shorter than those in the control group on the 2nd, 4th, and 5th training days （P 〈0.05 or P 〈0.01 ）, and was significantly shorter than those in the anesthesia group on the 4th and 5th training days （ P 〈 0.05 or P 〈

关 键 词：小鼠 手术应激 皮质酮 水迷宫学习 

分 类 号：R614.2[医药卫生—麻醉学]