吡咯喹啉醌对γ射线照射后AGS细胞抗氧化力的影响  

Effect of pyrroloquinoline quinine on anti-oxidative competence of AGS cell radiated by γ-ray

作　　者：邱秀芹[1,2] 刘春亮[1] 徐岚[1] 赵俊宇[1] 吴士良[1] 

机构地区：[1]苏州大学医学部生物化学与分子生物学教研室生化工程研究所,江苏苏州215123 [2]苏州卫生职业技术学院,江苏苏州215009

出　　处：《江苏大学学报（医学版）》2009年第4期293-295,301,共4页Journal of Jiangsu University：Medicine Edition

基　　金：国防基础科研重点基金资助子项目(A3820060130)

摘　　要：目的:探讨吡咯喹啉醌(pyrroloquinoline quinone,PQQ)对60Coγ射线照射细胞清除自由基能力。方法:培养AGS细胞至对数期,实验分为6组:正常培养未照射组、单纯照射组、PQQ培养12 h未照射组、PQQ培养4 h未照射组、PQQ培养12 h后照射组、PQQ培养4 h后照射组。60Coγ照射,剂量8Gy。照射后6,12,24 h测定细胞总抗氧化力(T-AOC),SOD,MDA含量。结果:与正常未照组比较,照射后6,12,24 h单纯照射组SOD,T-AOC显著降低(P<0.05),MDA显著升高(P<0.01);与单纯照射组比较:PQQ培养的照射组SOD,T-AOC显著升高(P<0.01),除照射后6 h外MDA显著降低(P<0.05)。结论:PQQ可通过增强细胞抗氧化能力,降低氧化水平,从而对辐射细胞起保护作用。Objective： To investigate PQQ scavenging free radicals in cells by γ-ray radiation. Methods： AGS cells were cultivated. There were 6 groups in our experiment： the normal group, simply irradia- ted group, nonirradiated group treated with PQQ by 12 hours, nonirradiated groups treated with PQQ by 4 hours, irradiated groups treated with PQQ by 12 hours, irradiated groups treated with PQQ by 4 hours. The radiation was ^60Coγ-rays with the dose of 8Gy. After irradiated 6 h, 12 h, 24 h, the cells were measured of theses level： total antioxidation competence （T-AOC）, SOD, MDA. Results： The level of T-AOC and SOD decreased （ P 〈 0.05 ）, and the level of MDA increased （ P 〈 0.01 ） in simply irradiated groups after irradiated 6 h, 12 h, 24 h,in comparison with the normal group. The level of T-AOC and SOD increased （P 〈 0.01 ） in irradiated groups treated with PQQ in comparison with the simply irradiated group; and the level of MDA decreased （P 〈 0.05 ）. Conclusion： PQQ can protect radiated cells, by strengthening anti-oxidative competence and lessening the oxidation of cells.

关 键 词：AGS细胞 吡咯喹啉醌 抗氧化力 

分 类 号：R454[医药卫生—治疗学] R914[医药卫生—临床医学]