外源nfeC基因导入快生型大豆根瘤菌菌株HN01的行为分析  

Behaviors of Exogenous nfeC Gene in Sinorhizobuum Fredii Strain HN01

作　　者：马庆生[1] 武波[1] 唐东阶[1] 柏学亮[1] 何曙光[1] 周刚林[2] 

机构地区：[1]广西大学农业分子遗传农业部重点开放实验室 [2]广西大学生物化学教研室

出　　处：《复旦学报（自然科学版）》1998年第4期445-449,共5页Journal of Fudan University：Natural Science

基　　金：国家科委八六三高科技项目;广西自然科学基金

摘　　要：nfeC基因是从慢生型大豆根瘤茵中克隆到的、与竞争结瘤有关的基因．将含nfeC基因的质粒pGXN100导入快生型大豆根瘤菌菌株HN01后，构建了重组快生型大豆根瘤菌GX301．与HN01相比较，GX301的竞争结瘤能力增强．It had been shown that nfeC gene was essential for nodulation competitiveness of Bradyrhizobium joponicum. A Sinorhizobium fredii strain GX301 was constructed by introducing the plasmid pGXN100 containing the nfeC gene into the Sinorhizobium fredii strain HN01. It was Shown that GX301 can enhance the compentitiveness nodulation compared with the HN01.

关 键 词：大豆根瘤菌 结瘤效率基因 竞争结瘤 重组 

分 类 号：S154.381[农业科学—土壤学] Q784[农业科学—农业基础科学]