激素对小麦和小黑麦杂交后代花药培养和染色体加倍的影响  

The Effect of Hormone on Anther Culture and Chromosome Doubling of Triticum×Triticale Hybrid Progeny

作　　者：李文卓[1] 郝峥嵘[1] 崔建平 沈大棱[1] 吴根法 陶建秋 

机构地区：[1]复旦大学 [2]宝山生物技术中心

出　　处：《复旦学报（自然科学版）》1998年第4期573-576,共4页Journal of Fudan University：Natural Science

基　　金：上海市科委基金

摘　　要：用不同培养基对小麦和小黑麦杂交后代进行花药培养，发现含2．4－D和6BA（V（2．4－D）：V（68A）＝2：1）的培养基上形成的愈伤组织，其再分化苗的绿苗率和染色体加倍年远高于其他培养基．说明杂交后代对不同的细胞分裂素的敏感度是不同的，而6BA作为一种细胞分裂素，其作用也不仅仅是有助于愈伤组织的再分化．这对花药培养进一步的理论研究会有所帮助．Anther culture of progenies from Triticum×Triticale hybird was carried out on C17 medium containing different kinds and levels of plant hormones. The data showed that the quality of callus, as well as the rate of differenciated green plants and frequency of chromosome doubling, on the medium containing 2. 4-D 2 mg/L and 6BA 1 mg/L was obviously higher than that on other medium. It was revealed that hybrid progeny showed different sensitivity to different plant cytomins. As a plant hormone, 6BA was functional not only in callus differenciation. These results are useful in further theoretical research of anther culture.

关 键 词：小麦 小黑麦 花药培养 染色体加倍 激素 杂交 

分 类 号：S512.103.2[农业科学—作物学]