四逆散对运动性疲劳大鼠海马突触素的调节作用  

Expression of Synaptohydin in Hippocampus of Exercise-fatigue Rats by Sinisan

作　　者：李维[1] 孔烈[1] 于爽[1] 郑硕[1] 徐铭谦[1] 张蓉[2] 刘燕[1] 刘晓兰[1] 李峰[1] 

机构地区：[1]北京中医药大学基础医学院,北京市100029 [2]北京第二炮兵总医院,北京市100088

出　　处：《中国康复理论与实践》2009年第8期739-741,共3页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家基础科学人才培养基金资助(c20070103)

摘　　要：目的观察四逆散对运动性疲劳大鼠海马内突触素表达的影响及其对学习记忆能力的影响。方法将大鼠分成正常组,模型组和治疗组,运用力竭游泳方法制造运动性疲劳大鼠模型,造模同时给予治疗组四逆散灌胃10日后,用被动回避实验观测大鼠学习记忆能力的改变,采用免疫组化技术观测大鼠海马CA1区突触素的表达变化。结果①各组大鼠的避暗潜伏期/避暗错误次数:正常对照组为(144.25±57.14)s/(0.50±0.80),模型组为(73.00±61.96)s/(1.67±1.15),四逆散组为(166.17±47.92)s/(0.38±0.49);②各组大鼠海马突触素平均光密度值:正常对照组为(0.2636±0.10654),四逆散组为(0.2555±0.16338),高于模型组为(0.0474±0.1837)(P<0.05);③突触素免疫组化染色:正常对照组海马神经细胞周围突触素阳性颗粒密集,染色深,高于四逆散组和模型组。正常对照组的海马神经细胞排列整齐、紧密;模型组大鼠海马细胞排列松散,不紧密;四逆散组海马排列介于正常对照组和模型组之间。结论四逆散具有改善运动性疲劳大鼠学习记忆的能力,具有增加运动性疲劳大鼠海马突触素表达的作用。Objective To observe the effect of Sinisan on the learning and memorial ability and the expression of synaptohydin in the hippocampus of exercise-fatigue rats. Methods Rats were made into the fatigue model by Exhaustive Swimming. Learning and memorial ability of the rats were observed by moving-back with current stimulation experiment; expression of synaptohydin protein in hippocampus of exercise-fatigue rats treated with Sinisan for 10 days were detected by immunohistoehemistry technique and compared to control group and model group. Results The moving-back time and the error times of the exercise-fatlgue rats as follows： model group（73.00±61.96） s/（1.67±1.15）, control group（144.25±57.14） s/（0. 50±0.80）,Sinisan group （166.17±47.92） s/ （0. 38±0.49）. Comparison of mean optical density of synaptohydin protein in hippocampus of rats in different groups as follows： control group （0. 2636±0. 10654） and Sinisan group （0. 2555±0. 163380） were higher than the model group （0. 0474±0. 1837）（P〈0.05）. The synaptohydin staining results showed that synaptohydin-positive granules around the neuron in hippocampus of rats in control group were the darkest and densest, the Sinisan group were darker than the model group rats. Conclusion Sinisan could improve the learning and memorial ability and increase the synaptohydin protein in the hippocampus of the exercise-fatigue rats.

关 键 词：运动性疲劳 四逆散 海马 突触素 

分 类 号：R872.7[医药卫生—运动医学]