重组Pax6腺病毒对神经细胞周期的影响  

Effects of Pax6-adenovirus Transfection on the Cell Cycle of SH-SY5Y Cells

作　　者：扈启宽[1] 李美育[2] 滕洒洒[2] 白洁[1] 

机构地区：[1]宁夏医科大学基础医学院生理学教研室自治区颅脑疾病重点实验室,银川750004 [2]北京大学干细胞研究中心,北京100191

出　　处：《宁夏医科大学学报》2009年第4期423-425,共3页Journal of Ningxia Medical University

基　　金：宁夏自然科学基金NZ0777;NZ0778

摘　　要：目的检测Pax6是否直接调控细胞周期。方法重组Pax6腺病毒转染SH-SY5Y神经细胞系使Pax6过表达,用流式细胞术检测细胞周期变化。结果SH-SY5Y神经细胞系Pax6过表达未影响细胞周期G0/G1、G2/M、S期参数,经统计后与对照组无统计学意义。结论转染重组Pax6腺病毒不影响SH-SY5Y细胞周期。Objective To explore that whether Pax6 transfection would have any effects on the cell cycle of neural cells. Methods The SH-SY5Y cells by adenovirus expressing Pax6 were transfected, and the DNA contents were determined by flow cytometry. Results The percent of cells in the G0/G1, G2/M and S phase had not changed after Pax6 transfection. Conclusion Overexpression of Pax6 have no significant effect on the cell cycle of SH-SY5Y cells.

关 键 词：PAX6 腺病毒 细胞周期 

分 类 号：R341[医药卫生—基础医学]