D-氨基葡萄糖盐酸盐诱导淋巴细胞增殖及其机制  

Effect of D-glucosamine hydrochloride on lymphocytes proliferation and its mechanism

作　　者：曹秀明[1] 马娇[1] 万晓峰[1] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心药物所抗肿瘤天然药物教育部工程研究中心,黑龙江哈尔滨150076

出　　处：《中国生化药物杂志》2009年第4期235-238,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：黑龙江省科技厅自然科学基金(D2007-45)

摘　　要：目的研究D-氨基葡萄糖盐酸盐(GAH)对小鼠淋巴细胞增殖及其作用机制。方法利用荧光染料CFSE为细胞标记,研究GAH对小鼠淋巴细胞增殖的影响。激光共聚焦显微镜、流式细胞仪分别检测GAH对细胞内Ca2+浓度、钙调蛋白(CaM)表达的影响。结果GAH可使淋巴细胞增殖。加入GAH 2,4,6 h后,细胞内Ca2+浓度与对照组相比显著升高。GAH作用72 h后,细胞内CaM阳性表达细胞的百分率与对照组相比显著增加,由(61.80±0.97)%增至(79.21±0.95)%,说明GAH能够诱导淋巴细胞内CaM表达增加。结论GAH能够提高淋巴细胞内Ca2+浓度,促进CaM表达,触发Ca2+信号通路,活化淋巴细胞增殖。Purpose To investigate the effect of D-glucosamine hydrochloride（GAH） on lymphoeytes proliferation and its mechanism. Methods Fluorochrome label, scanning microscope and flow cytometry were used to investigate the effects of GAH on lymphoeytes proliferation, intraeellular Ca^2＋ concentration and expression of calmodulin（ CaM）. Results GAH can induce lymphoeytes proliferation 2,4 and 6 h after being treated with GAH, and intracellular Ca^2＋ eoncentration was stepped up 72 h after being treated with GAH. The percentage of intracellular calmodulin expression was increased by GAH. Conclusion GAH ean increase the intracellular Cae ＋ , incourage ealmodulin expression and trigger Ca^2＋ signal pathway and aetivate lymphoeytes proliferation.

关 键 词：D-氨基葡萄糖盐酸盐 淋巴细胞增殖 CA2+ 钙调蛋白 

分 类 号：R965[医药卫生—药理学]