叶酸-白蛋白纳米粒偶联荧光素制备工艺的研究  

Study on preparation of folate-conjugated fluorescein-loaded album nanoparticles

作　　者：曹洁[1] 刘飞[1] 顾月清[1] 

机构地区：[1]中国药科大学生命科学与技术学院,江苏南京210009

出　　处：《中国生化药物杂志》2009年第4期251-254,共4页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的研究叶酸-白蛋白纳米粒(叶酸-BSA纳米粒)偶联荧光素的制备工艺。方法制备叶酸-BSA纳米粒偶联物,将所得偶联物物理包合荧光素,通过透析将大部分未包裹的荧光素除去,并用葡聚糖凝胶柱色谱法进一步分离纯化,用紫外分光光度法测定荧光素上载效率。结果通过物理包合可以成功将荧光素包裹到叶酸-BSA纳米粒内,荧光素上载效率为6.4%。结论通过研究叶酸-BSA纳米粒包裹荧光素的制备工艺,可为进一步利用叶酸-BSA纳米粒偶联物包裹近红外荧光染料,对肿瘤体内在位监测奠定了基础。Purpose To study on the preparation of folate-eonjugated fluoreseein-loaded album nanopaticles. Methods The bovine serum albumin nanoparticles （BSANPs） were prepared by desolvation method and chemical cross-linking with glutaraldehyde. The aetivated folie acid （N-hydroxysueeinimide ester of folio aeid） was conjugated to the surface of BSANPs via the amino groups. Then fluoreseein was incorporated of into the folate-conjugated BSANPs, purifying with Sephadex G-50 eolumn. The extent of fluorescein loaded on the folate-BSANPs was determined by UV speetrophotometie analysis. Results The fluoreseein was sueeessfully loaded into the folate-eonjugated album nanopatieles, while the entrapment rate of fluoreseein is 6.4%. Conclusion Folate-conjugated fluorescein-loaded album nanopaticles were achieved, which represent a new future of loading the near-infrared fluorescein into folate-eonjugated album nanopaticles.

关 键 词：叶酸 白蛋白纳米粒 荧光素 SEPHADEX G-50葡聚糖凝胶 

分 类 号：R944.9[医药卫生—药剂学] TQ464.7[医药卫生—药学]