时间分辨荧光免疫法检测乙肝标志物的临床评价  

Clinical estimation of time resolved fluorescence immunoassay on detection of serum markers of hepatitis B virus

作　　者：邓爱玲 戴春梅[2] 

机构地区：[1]长沙市雨花区妇幼保健所,湖南长沙410014 [2]中南大学湘雅三医院检验科,湖南长沙410013

出　　处：《现代医药卫生》2009年第18期2763-2765,共3页Journal of Modern Medicine & Health

摘　　要：目的:探讨时间分辨荧光免疫分析技术(TRFIA)检测乙型肝炎血清学标志物(HBV-M)的临床意义。方法:用TRFIA和ELISA法对健康对照组115份血清和感染组210份血清的HBV-M进行测定。结果:用TRFIA和ELISA法检测HBsAg对照组和感染组均无差异;TRFIA检测HBsAb比ELISA法灵敏度高,可检出低浓度及过高浓度的HBsAb;TRFIA检测HBeAg比ELISA法特异性强,ELISA法易出现假阳性;TRFIA检测HBeAb和HBcAb与ELISA法相比对感染组差异有显著性。结论:TRFIA法在检测HBV-M是特异性强、敏感性高,其结果间接反映病毒的感染状况,同时也可为接种乙肝疫苗提供依据。HBeAg和HBsAg可作为监测HBV感染和复制的量化指标。动态监测HBV-M的量能够反映机体对病毒感染的免疫反应情况,可作为观察临床疗效的依据。Objective ：To estimate the clinical value of the time resolved fluoescence immunoassay（TRFIA） in detecting serum markers of hepatitis B virus （HBV-M）.Methods：We used the methods of TRFIA and ELISA to measure the HBV-M of 115 serum samples from control subjects and 210 serum samples from patients respectively.Results：There were no discrepancy in control subject and patients using the methods of TRFIA and ELISA to measure HBsAg;TRFIA has a higher sensitivity to HBsAb.TRFIA was able to detect excessively low or high eoneentraction of HBsAb.Also it had a higher speciality to HBeAg, while ELISA had a higher rate of false positive relatively.There was statistical difference between ELISA and TRFIA in detecting HBeAb and HBcAb.Conclusion：TRFIA has a higher sensitivity and speciality in detecting HBV-M, which could reflect the HBV infection condition indirectly and provide a reliable guide on injecting HBV vaccine. Monitoring the developmemt of HBV-M content provides a good reflection on our body＇s reaction to virus infecting,whieh can be used as the evidence for observing clinical course of disease and curative effect.

关 键 词：乙型肝炎病毒标志物 酶联免疫吸附试验 时间分辨荧光免疫分析技术 

分 类 号：R446[医药卫生—诊断学]