水稻液泡膜H^+-ATPase诱导性冷失活机制研究  

STUDY ON MECHANISM OF INDUED COLD INACTIVATION OF TONOPLAST H + ATPASE iN RICE

作　　者：李松[1] 李如亮[1] 祝雄伟[1] 李曼铭[1] 王延枝[1] 

机构地区：[1]武汉大学生命科学学院

出　　处：《武汉大学学报（自然科学版）》1998年第4期489-492,共4页Journal of Wuhan University(Natural Science Edition)

基　　金：国家自然科学基金

摘　　要：以水稻液泡膜Ｈ＋－ＡＴＰａｓｅ为研究对象，探讨了造成诱导性冷失活现象的机制．运用ＭＣ５４０荧光对诱导性冷失活过程中的膜脂状态研究表明，冷失活处理后膜脂的有序程度大幅度下降（ＭＣ５４０荧光发射增强５６％），并且通过与常温下诱导物（Ｍｇ２＋、ＡＴＰ）扰动膜脂的效应比较，证明诱导物在冷失活条件下能诱导膜脂有序性迅速丧失，其后才出现酶活丧失．根据实验结果和膜脂－膜蛋白相互作用关系。the mechanism of cold inactivation on rice tonoplast H + ATPase is studied,paying special attention on the influence of tonoplast lipid.MC540 is used to detect the packing order of tonoplast lipid,the result showed this order is destroyed in a great level(fluorescence emission increased by 56%).The same influence of the inducers(Mg 2+ ,ATP)is relatively weaker.This result demonstrated the inducers can induce the destruction of the order of tonoplast lipid,then,the enzyme activity declined.According to the above results and the lipid protein interaction,we consider that the tonoplast lipid plays an improtant role in cold inactivation of H + ATPase.

关 键 词：V型 H^+-ATP酶 冷失活 液泡膜 水稻 

分 类 号：S511.01[农业科学—作物学]