茶黄素降低缺血再灌注损伤大鼠心室肌细胞内游离钙浓度的研究  

Study of theaflavin on reducing intracellular free calcium concentration in ventricular myocytes in rats with ischemia/reperfusion injury

作　　者：马慧娟[1] 马会杰[1] 张金楠 刘宜先[1] 关玥[1] 张翼[1] 

机构地区：[1]河北医科大学 [2]解放军93670部队

出　　处：《现代中西医结合杂志》2009年第25期3024-3025,3029,共3页Modern Journal of Integrated Traditional Chinese and Western Medicine

基　　金：河北省二零零八医学科学研究重点课题计划(08266);2007年度河北省科学技术研究与发展指导计划(07276174)

摘　　要：目的研究茶黄素对大鼠心室肌细胞内游离钙浓度([Ca2+]i)的影响并探讨其可能机制。方法在正常台氏液和模拟缺血液中,用激光共聚焦显微镜探测[Ca2+]i,结果用相对荧光强度表示。结果茶黄素(20μmol/L)对正常台氏液中心室肌细胞内[Ca2+]i有影响,但可降低模拟缺血液中心室肌细胞[Ca2+]i的增加。应用钠钙交换体抑制剂NiCl2(100μmol/L)可模拟茶黄素(20μmol/L)在模拟缺血液中的作用。预先应用肌浆网钙泵抑制剂Thapsigar-gin(0.5μmol/L)可大部分取消茶黄素(20μmol/L)在模拟缺血液中的作用。结论茶黄素可通过抑制钠钙交换体转运和增强肌浆网钙泵活动,抑制缺血再灌注损伤中细胞[Ca2+]i的增加。Objective It is to explore the effects of theaflavin on intracellular calcium concentration [Ca2＋ ]1 in rat ventricular myocytes and to discuss the possible mechanisms. Methods [ Ca2 ＋ ]1 in normal Tyrode＇s solution and simulated ischemia solution was detected by laser confocal microcopy and the result was represented by relative fluorescent intensity. Results Theaflavin （20 μmol/L） didn＇t affect the [Ca2 ＋ ]1 of ventricular myocytes in normal Tyrode＇s solution, but reduced that in simulated isehemia ,solution. Pretreatment with the sodium-calcium exchanger inhibiter NiCI2 （100 μmol/L） had similar effect as theaflavin （20 〉mol/L）in simulated ischemia solution. Pretreatment with Thapsigargin （0.5 μmol/L）, a specific SR [Ca2 ＋ ]1 pump ATPase inhibitor, mostly abolished the effects of theaflavin （20μmol/L） in simulated ischemia solution. Conclusion Theaflavin may reduce the [Ca2＋]1 in ventrieular myocytes after ischemia/reperfusion injury through inhibiting sodium-calcium exchanger and improving the activity of SR [Ca2 ＋]1-pump ATPase.

关 键 词：茶黄素 心室肌细胞 细胞内钙 钠钙交换体 

分 类 号：R-332[医药卫生]